Departement Planten Systeem Biologie, VIB, Universiteit Gent, Technologiepark 927, B-9052 Gent, Belgium.
Mol Plant Pathol. 2005 May 1;6(3):321-5. doi: 10.1111/j.1364-3703.2005.00283.x.
SUMMARY Promoter activity of ABI3 and of three LEA genes was monitored in Arabidopsis transgenics infected with Heterodera schachtii and Meloidogyne incognita. ABI3::GUS expression was induced (in four different promoter deletion constructs) during early infection stages with H. schachtii. Similar GUS expression patterns, though slightly later in time compared with ABI3, were observed for one of the LEA promoter constructs, whereas the other two were not induced by H. schachtii. Expression was mainly observed in the syncytia. In contrast, little or no reporter gene expression was observed upon infection with M. incognita. The data suggest a role for ABI3 during the formation and active growth of the syncytium and demonstrate a marked difference between syncytium and giant cell ontogenesis.
在感染麦根长足夜蛾和马铃薯金线虫的拟南芥转基因植株中，监测了 ABI3 和三个 LEA 基因的启动子活性。ABI3::GUS 表达在 H. schachtii 的早期感染阶段被诱导（在四个不同的启动子缺失构建体中）。与 ABI3 相比，尽管时间稍晚，但对 LEA 启动子构建体之一观察到了类似的 GUS 表达模式，而另外两个则未被 H. schachtii 诱导。表达主要观察到在合胞体中。相比之下，感染麦根长足夜蛾时很少或没有观察到报告基因表达。这些数据表明 ABI3 在合胞体的形成和活跃生长过程中起作用，并证明了合胞体和巨型细胞发生之间存在显著差异。
