Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
Bioresour Technol. 2010 Nov;101(21):8016-23. doi: 10.1016/j.biortech.2010.05.025. Epub 2010 Jun 20.
The objective of this study was to investigate the potential ability of decolorizing Reactive Dark Blue K-R by the synergism of fungal strain Penicillium sp. QQ and bacterial strain Exiguobacterium sp. TL. The optimal conditions for decolorization determined by response surface methodology (RSM) were as follows: 12.5% (w/v) of both strains QQ and TL, 0.19% (w/v) of glucose, 0.09% (w/v) of yeast extract, pH7.0, 37 degrees C and 200mg/L of initial dye concentration. It was shown that about 97% of 200mg/L K-R could be decolorized within 24h under these conditions. Besides, strains QQ and TL co-cultures could decolorize 10 kinds of azo dyes effectively and be still 60% color removal when salinity increased to 15%. And most of the tested metal ions showed little influence on the decolorization except for Ni(2+). The results demonstrated that strains QQ and TL co-cultures could be useful for the practical bioremediation.
本研究旨在探讨真菌菌株青霉 QQ 和细菌菌株极端小球菌 TL 的协同作用对活性深蓝 K-R 的潜在脱色能力。响应面法（RSM）确定的最佳脱色条件如下：菌株 QQ 和 TL 的浓度为 12.5%（w/v），葡萄糖浓度为 0.19%（w/v），酵母提取物浓度为 0.09%（w/v），pH 值为 7.0，温度为 37°C，初始染料浓度为 200mg/L。结果表明，在这些条件下，200mg/L K-R 的约 97%可在 24 小时内脱色。此外，菌株 QQ 和 TL 共培养物可以有效脱色 10 种偶氮染料，当盐度增加到 15%时，仍有 60%的颜色去除率。并且除 Ni(2+)外，大多数测试金属离子对脱色几乎没有影响。结果表明，菌株 QQ 和 TL 共培养物可用于实际生物修复。
