Nagai M, Ikeda K, Tasaka T, Irino S
First Department of Internal Medicine, Kagawa Medical School, Japan.
Leukemia. 1991 Jun;5(6):462-7.
Southern blot analysis was employed to analyze the structural alterations of the c-myc oncogene in genomic DNA derived from tumor specimens of 35 adults with pathologically classified and immunophenotyped non-AIDS-related, non-Hodgkin's lymphoma in Japan. In this study, seven cases (20%), including one peripheral T-cell lymphoma and six B-cell lymphomas of various histological types, were demonstrated to have additional c-myc fragments. An interesting feature is that c-myc rearrangements were found in three out of eight primary gastrointestinal lymphomas. Analyses with several restriction enzymes revealed that the breakpoints in these cases were clustered in a region spanning the first exon, first intron and nearby 5'-flanking sequences of the c-myc gene, suggesting that the alteration of this region may represent an important molecular event in activating the oncogenic potential of the c-myc gene.
采用Southern印迹分析，对来自日本35例经病理分类和免疫表型分析的非艾滋病相关、非霍奇金淋巴瘤成年患者肿瘤标本的基因组DNA中c-myc癌基因的结构改变进行分析。在本研究中，7例（20%），包括1例外周T细胞淋巴瘤和6例不同组织学类型的B细胞淋巴瘤，被证实有额外的c-myc片段。一个有趣的特征是，在8例原发性胃肠道淋巴瘤中有3例发现了c-myc重排。用几种限制性内切酶进行分析显示，这些病例中的断点聚集在一个跨越c-myc基因第一外显子、第一内含子和附近5'侧翼序列的区域，这表明该区域的改变可能代表激活c-myc基因致癌潜能的一个重要分子事件。
