Departamento de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, Madrid, Spain.
Phytother Res. 2011 Jan;25(1):25-32. doi: 10.1002/ptr.3202.
Aqueous extracts and a semipurified fraction obtained by methanol extraction and column chromatography were isolated from Ganoderma lucidum [Ganoderma lucidum (Curtis) P. Karst.; Ganodermataceae Donk] and their effects on interleukin 3-dependent lymphoma cells (DA-1) were studied. Cell viability was reduced by the action of unboiled aqueous extract and by the methanol-extracted column-chromatography semipurified fraction, producing DNA fragmentation in DA-1 cells. Treatments with aqueous extracts showed increments of Bax after 13 h, increments of p53 and Mdm2 after 19 h and a reduction of these three proteins after 24 h. The methanol-extracted semipurified fraction also induced increments of p53 and Mdm2 factors at 19 h with a reduction after 24 h. The methanol-extracted column-chromatography semipurified fraction from Ganoderma lucidum produced minor changes in the level of Akt after treatments for 19 h in DA-1 cells with a slight reduction in the levels of NFkB-p65 factor. Both the unboiled aqueous extract and the methanol-extracted column-chromatography semipurified fraction produced cleavage of inactive caspase 3, as a clear indication of induction of apoptosis by compounds present in Ganoderma lucidum.
从灵芝（Ganoderma lucidum（Curtis）P. Karst.；灵芝科 Donk）中分离出甲醇提取和柱层析获得的水提取物和半纯化级分，并研究其对白细胞介素 3 依赖性淋巴瘤细胞（DA-1）的影响。未煮沸的水提取物和甲醇提取的柱层析半纯化级分的作用降低了 DA-1 细胞的活力，并导致 DNA 片段化。水提取物处理后 13 小时 Bax 增加，19 小时 p53 和 Mdm2 增加，24 小时后这三种蛋白质减少。甲醇提取的半纯化级分也在 19 小时诱导 p53 和 Mdm2 因子增加，24 小时后减少。甲醇提取的柱层析半纯化级分在 DA-1 细胞中处理 19 小时后，Akt 水平仅略有变化，NFkB-p65 因子水平略有降低。未煮沸的水提取物和甲醇提取的柱层析半纯化级分均使无活性 caspase 3 发生裂解，这清楚地表明灵芝中存在的化合物诱导了细胞凋亡。
