Department of Biology, Graduate School of Science, Chiba University, Chiba 263-8522, Japan.
Dev Dyn. 2010 Aug;239(8):2208-18. doi: 10.1002/dvdy.22354.
We developed a culture method for detecting repulsion among epithelial lobules during branching morphogenesis in mouse submandibular glands. Three epithelia were placed at each vertex of an imaginary triangle apart but near enough to meet with one another if each of them expands radially during the culture period. No repulsion was observed following cultivation with neuregulin 1 and lysophosphatidic acid; the epithelia actively branched and nearly contacted one another in the triangle's center. In contrast, strong repulsion was observed among the epithelia cultured with fibroblast growth factor 1 (FGF1), which exhibited less branching and moved away from the center. The localization of DiI- (1,1', di-octadecyl-3,3,3',3',-tetramethylindo-carbocyanine perchlorate) and BrdU- (5-bromodeoxyuridine) labeled cells in the cultures exposed to FGF1 indicated that the cells were unable to move and proliferate in the center. SB431542, an inhibitor of transforming growth factor-beta (TGFbeta) signaling, was unable to abolish this repulsion, suggesting that TGFbetas will not probably act as repellants in this case.
我们开发了一种培养方法，用于检测在小鼠下颌下腺分支形态发生过程中上皮小叶之间的排斥。在想象中的三角形的每个顶点放置三个上皮，彼此之间分开但足够近，如果它们在培养期间都径向扩展，则可以彼此接触。在神经调节蛋白 1 和溶血磷脂酸存在下培养时，没有观察到排斥现象；上皮细胞在三角形的中心积极分支并几乎相互接触。相比之下，在与成纤维细胞生长因子 1（FGF1）培养的上皮之间观察到强烈的排斥，FGF1 表现出较少的分支并且远离中心移动。暴露于 FGF1 的培养物中 DiI（1,1'，二十八烷基-3,3,3'，3'，-四甲基吲哚碳菁高氯酸盐）和 BrdU（5-溴脱氧尿苷）标记细胞的定位表明，这些细胞无法在中心移动和增殖。转化生长因子-β（TGFβ）信号的抑制剂 SB431542 不能消除这种排斥，表明在这种情况下 TGFbetas 可能不会作为排斥物起作用。
