School of Life Sciences, Shanghai University, Shanghai, People's Republic of China.
J Neurosci Res. 2010 Oct;88(13):2775-86. doi: 10.1002/jnr.22454.
During cerebellar development, the chemokine stromal cell-derived factor-1 alpha (SDF-1 alpha) has been shown to play an important role in recruiting cells from the upper rhombic lip (URL) and external granule cell layer (EGL). However, its function in cerebellar development is still poorly understood. Our results have demonstrated that SDF-1 is necessary for EGL development, and URL cells stream to the SDF-1 source in vitro. Results of embryonic URL explant assays and transwell assays indicated that SDF-1 induces neural cell migration from the URL region in chemotactic and chemokinetic responses. The time-lapse results showed that the migration speed of granule cell progenitors out of the URL was accelerated by the addition of recombinant SDF-1 alpha. Collectively, our study shows that SDF-1 increases the motility of URL cells in the absence of a gradient and promotes the migration of granule cell progenitors during cerebellar development.
在小脑发育过程中，趋化因子基质细胞衍生因子-1 ɑ（SDF-1ɑ）已被证明在招募来自上菱唇（URL）和外颗粒细胞层（EGL）的细胞方面发挥重要作用。然而，其在小脑发育中的功能仍知之甚少。我们的研究结果表明 SDF-1 对于 EGL 发育是必需的，并且 URL 细胞在体外向 SDF-1 来源处流注。胚胎 URL 外植体分析和 Transwell 分析的结果表明，SDF-1 以趋化和趋动运动方式诱导来自 URL 区的神经细胞迁移。延时结果表明，添加重组 SDF-1ɑ可加速颗粒细胞前体细胞离开 URL 的迁移速度。总之，我们的研究表明，SDF-1 在没有梯度的情况下增加 URL 细胞的迁移能力，并促进小脑发育过程中颗粒细胞前体细胞的迁移。
