Kalluri Haviryaji S G, Dempsey Robert J
Department of Neurological Surgery, University of Wisconsin, Madison, Wisconsin 53792, USA.
Neuroreport. 2010 Jul 14;21(10):700-3.
We examined the effect of tricyclodecan-9-yl-xanthogenate (D609), a phosphatidylcholine-specific phospholipase C inhibitor, on the proliferation of adult neural progenitor cells in vitro. D609 (100 microM) decreased the proliferation of neural progenitor cells as measured by the proliferation assay, bromodeoxyuridine incorporation, and cell counting without causing cell death. Immunoblot analysis showed a decrease in the expression of cyclin D1 and phosphorylation of retinoblastoma after the incubation of cells with D609. These results suggest that D609 inhibits the proliferation of neural progenitor cells by reducing the cyclin D1 content resulting in decreased phosphorylation of retinoblastoma and subsequent inhibition of cell division.
我们研究了磷脂酰胆碱特异性磷脂酶C抑制剂三环癸烷-9-基黄原酸酯（D609）对成年神经祖细胞体外增殖的影响。通过增殖试验、溴脱氧尿苷掺入和细胞计数测定，D609（100微摩尔）可降低神经祖细胞的增殖，且不会导致细胞死亡。免疫印迹分析显示，细胞与D609孵育后，细胞周期蛋白D1的表达和视网膜母细胞瘤的磷酸化水平降低。这些结果表明，D609通过降低细胞周期蛋白D1含量来抑制神经祖细胞的增殖，从而导致视网膜母细胞瘤磷酸化水平降低，进而抑制细胞分裂。
