Dokuz Eylul University, Izmir, Turkey.
Neoplasma. 2010;57(5):465-72. doi: 10.4149/neo_2010_05_465.
We studied the promoter methylation status and expression levels of P16 and CDH1 genes in breast cancer and their adjacent normal tissues with normal control breast tissues, to correlate with their histopathological parameters. Hundred twenty four samples (tumor and adjacent nonmalignant tissues) from 62 breast cancer patients and 4 normal control breast tissues were included in the study. We used methylation specific PCR to evaluate methylation status and quantitative RT-PCR to measure the gene expression levels. Methylation incidence of P16 gene and CDH1 gene in tumor tissues were 24.2 % and 33.9 %, respectively. CDH1 and P16 gene were not methylated in normal control tissues. CDH1 underexpression is found to be significant in correlation with advanced stage, histologic type, high tumor grade and lymph node involvement. P16 expression is found not to be significantly related with any histopathological parameters. But 60% of cases which overexpresses P16 were estrogen negative, and 40% of them were histologic grade 3. Both P16 and CDH1 had different expression levels in tumor tissues compared to the adjacent normal tissues and in adjacent normal tissues compared to the normal non-tumor tissues.
我们研究了乳腺癌及其相邻正常组织与正常对照乳腺组织中 P16 和 CDH1 基因的启动子甲基化状态和表达水平，并将其与组织病理学参数相关联。本研究共纳入 62 例乳腺癌患者的 124 个样本（肿瘤和相邻非恶性组织）和 4 个正常对照乳腺组织。我们使用甲基化特异性 PCR 评估甲基化状态，并用定量 RT-PCR 测量基因表达水平。肿瘤组织中 P16 基因和 CDH1 基因的甲基化发生率分别为 24.2%和 33.9%。正常对照组织中 CDH1 和 P16 基因未发生甲基化。CDH1 表达下调与晚期、组织学类型、高肿瘤分级和淋巴结受累显著相关。P16 表达与任何组织病理学参数均无显著相关性。但 60%的 P16 过表达病例为雌激素阴性，40%为组织学分级 3 级。与相邻正常组织相比，P16 和 CDH1 在肿瘤组织中的表达水平不同，与正常非肿瘤组织相比，P16 和 CDH1 在相邻正常组织中的表达水平也不同。
