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黄瓜花叶病毒亚基因组 RNA4A 启动子的核心启动子。

Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A.

机构信息

Department of Biology, Indiana University, Bloomington, IN 47405, USA.

出版信息

Mol Plant Pathol. 2002 Jan 1;3(1):43-52. doi: 10.1046/j.1464-6722.2001.00089.x.

DOI:10.1046/j.1464-6722.2001.00089.x
PMID:20569307
Abstract

summary The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3' of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3' of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.

摘要

摘要

本研究采用模板依赖性 RNA 合成分析和核心启动子 RNA 变体，对黄瓜花叶病毒（CMV）RNA4A 起始的核心亚基因组启动子进行了体外表征。体内使用的胞苷酸（T1）特异性起始所需的最小序列由 T1 下游的 31 个核苷酸（nt）和 13 个 nt 模板序列组成。该 44nt RNA 被发现提供了三个元件，这些元件有助于 CMV 复制酶有效起始 RNA4A 的合成：T1 下游的茎环二级结构、富含腺嘌呤和尿嘧啶的模板序列，以及未配对的 T1 序列。

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