Department of Molecular, Cellular and Developmental Biology, OML 451, Yale University, PO Box 208104, New Haven, CT 06520-8104, USA.
Mol Plant Pathol. 2002 May 1;3(3):167-72. doi: 10.1046/j.1364-3703.2002.00110.x.
Summary In this mini review we discuss recent advances in the understanding of the N gene-mediated resistance to tobacco mosaic virus (TMV). The tobacco N gene belongs to toll-interleukin-1 receptor homology/nucleotide binding/leucine rich repeat (TIR-NB-LRR) class of resistance genes. It encodes two transcripts, N(S) and N(L), by alternative splicing, both of which are required to confer resistance to TMV. The structure-function analysis of the N gene indicates that the TIR, NB and LRR domains are indispensable for its function. The N gene response is elicited by the C-terminal helicase domain of the 126 kDa TMV replicase protein. Tobacco N gene can also confer resistance to TMV in heterologous plants like tomato and Nicotiana benthamiana. Recent studies on N-mediated signalling suggest that EDS1, Rar1 and NPR1 genes play an important role in TMV resistance. Finally, we discuss current status of the N-mediated signal transduction and speculate directions for future work to understand N-TMV interaction.
在这篇迷你综述中，我们讨论了对烟草花叶病毒（TMV）的 N 基因介导的抗性的最新认识。烟草 N 基因属于 Toll-白细胞介素-1 受体同源物/核苷酸结合/亮氨酸丰富重复（TIR-NB-LRR）类抗性基因。它通过选择性剪接编码两种转录本，N(S)和 N(L)，两者都需要赋予对 TMV 的抗性。N 基因的结构-功能分析表明，TIR、NB 和 LRR 结构域对其功能是不可或缺的。N 基因的反应是由 126kDa TMV 复制酶蛋白的 C 端解旋酶结构域引发的。烟草 N 基因还可以在番茄和黄花烟等异源植物中赋予对 TMV 的抗性。最近对 N 介导的信号转导的研究表明，EDS1、Rar1 和 NPR1 基因在 TMV 抗性中起重要作用。最后，我们讨论了当前 N 介导的信号转导的现状，并推测了未来工作的方向，以了解 N-TMV 相互作用。
