牛干扰素刺激基因 15：感染牛传染性鼻气管炎病毒的胎牛肺细胞中的一种抗病毒和诱导蛋白。

Bovine ISG15: an antiviral and inducible protein in BIV infected fetal bovine lung cells.

机构信息

Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory of Microbial Function Genomics (Tianjin), College of Life Sciences, Nankai University; N0, 94, Rd, Weijin, Nankai District, Box, 300071, Tianjin, PR China.

出版信息

Virol J. 2010 Jun 23;7:134. doi: 10.1186/1743-422X-7-134.

DOI:10.1186/1743-422X-7-134

PMID:20569475

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2900246/

Abstract

Bovine ISG15 (bISG15) is an interferon inducible ubiquitin-like protein that is responsible for the establishment of early pregnancy in ruminant, understanding the properties of bISG15 capable of being inducible in fetal bovine lung (FBL) cells upon infection of bovine immunodeficiency virus (BIV) is of significant importance. In this study, we investigated the expression of bISG15 in poly I:C treated FBL cells. The increased expression of bISG15 was observed, and the inhibition of BIV replication was also detected in FBL cells. Elimination of bISG15 expression by small interfering RNA reversed the bISG15 mediated inhibition of BIV replication. These findings demonstrate that bISG15 plays an important role in inhibition of the BIV replication in FBL cells. Furthermore, real-time PCR and western blot assay revealed that bISG15's expression can also be induced in BIV infected FBL cells. Taken together, bISG15 is an antiviral and inducible protein in BIV infected FBL cells.

摘要

牛干扰素诱导的泛素样蛋白 15（bISG15）是一种在反刍动物中负责建立早期妊娠的干扰素诱导的泛素样蛋白，了解能够在牛免疫缺陷病毒（BIV）感染胎儿牛肺（FBL）细胞中诱导的 bISG15 的特性非常重要。在这项研究中，我们研究了 bISG15 在多聚肌苷酸处理的 FBL 细胞中的表达。观察到 bISG15 的表达增加，并检测到 FBL 细胞中 BIV 复制的抑制。通过小干扰 RNA 消除 bISG15 的表达，逆转了 bISG15 介导的 BIV 复制抑制。这些发现表明 bISG15 在抑制 FBL 细胞中的 BIV 复制中发挥重要作用。此外，实时 PCR 和 Western blot 分析显示，bISG15 的表达也可以在 BIV 感染的 FBL 细胞中诱导。总之，bISG15 是 BIV 感染的 FBL 细胞中的抗病毒和诱导蛋白。