Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Cell Stem Cell. 2010 Jun 4;6(6):521-31. doi: 10.1016/j.stem.2010.05.010.
Technology for the derivation, propagation, and characterization of pluripotent stem cell lines from the human embryo has undergone considerable refinement and improvement since the first published description of human embryonic stem cells in 1998. In particular, there has been extensive effort to optimize protocols and develop defined culture systems with a view toward future clinical applications of embryonic stem cell-derived products. Here, we review the current status of methodology for human embryonic stem cell derivation and culture, and we highlight the challenges that remain for workers in the field.
自从 1998 年首次发表人类胚胎干细胞的描述以来，人类胚胎多能干细胞系的衍生、传播和特征描述技术已经得到了相当大的改进和完善。特别是，人们已经做出了广泛的努力来优化方案和开发定义明确的培养系统，以期将胚胎干细胞衍生产品应用于未来的临床。在这里，我们回顾了人类胚胎干细胞衍生和培养的方法学的现状，并强调了该领域工作者仍然面临的挑战。
