Université de Lyon, Unité CRYOBIO, UPSP VetAgro Sup/ISARA-Lyon, Lyon, France.
Theriogenology. 2010 Sep 15;74(5):847-55. doi: 10.1016/j.theriogenology.2010.04.009. Epub 2010 Jun 8.
The cryopreservation of oocytes is valuable for the preservation of women's fertility and might also be an interesting tool to preserve animal genetic biodiversity but it is not often used because of the very poor fertility recovered after thawing, especially in rabbit species. The objective of our study was to evaluate the effect of slow-freezing and vitrification on the structural integrity of ovulated rabbit oocytes, their ATP contents, and their developmental competence. Results show that, whatever the method is used, cryopreservation has a dramatic effect on the metabolic integrity, the structural integrity, and the developmental ability of the oocytes. Vitrification and slow freezing both impair the rabbit oocytes viability after thawing but the processes act differently. Further studies are needed to improve the cryopreservation techniques in rabbit species. Moreover, we underlined that morphology and maintenance of the structural integrity of the oocytes are not suitable enough to assess the potential for further development of cryopreserved M(II) oocytes. The assessment of ATP metabolism allows efficient evaluation of the viability of the frozen or vitrified oocytes. It should be used in addition to parthenogenesis to better assess the potential for further development.
卵母细胞的冷冻保存对于保存女性生育能力具有重要意义，也可能是保存动物遗传生物多样性的一种有趣工具，但由于解冻后恢复的生育力非常差，尤其是在兔种中，因此并未广泛应用。我们的研究目的是评估慢速冷冻和玻璃化对排卵兔卵母细胞结构完整性、ATP 含量和发育能力的影响。结果表明，无论使用哪种方法，冷冻保存都会对卵母细胞的代谢完整性、结构完整性和发育能力产生巨大影响。玻璃化和慢速冷冻都会损害解冻后兔卵母细胞的活力，但作用方式不同。需要进一步研究来提高兔种的冷冻保存技术。此外，我们强调卵母细胞的形态和结构完整性的维持不足以评估冷冻保存 M(II)期卵母细胞进一步发育的潜力。ATP 代谢的评估可以有效地评估冷冻或玻璃化卵母细胞的活力。除了孤雌生殖外，它还应该被用来更好地评估进一步发育的潜力。
