Ghrelin 改善异丙肾上腺素诱导心力衰竭大鼠心肌能量代谢障碍。

Ghrelin improves disturbed myocardial energy metabolism in rats with heart failure induced by isoproterenol.

机构信息

Department of Physiology and Pathophysiology, Capital Medical University, Beijing 100069, China.

出版信息

J Pept Sci. 2010 Aug;16(8):392-402. doi: 10.1002/psc.1253.

PMID:20572026

Abstract

To explore the effects of ghrelin on disturbed myocardial energy metabolism during chronic heart failure (CHF). Rats were subcutaneously injected with isoproterenol (ISO) for 10 days with or without ghrelin for another 10 days. Enzyme immunoassay was to measure ghrelin concentrations. Compared with the control group, ISO-treated rats showed suppressed cardiac function with high ghrelin/GHS-R expressions. These rats also showed the decreases in food consumption and weight. The decreased levels of plasma glucose and myocardial glucogen, but the high lactate in blood and myocardium showed myocardial metabolic disturbance. Compared with the group given ISO alone, the rats with ghrelin (20 and 100 microg/kg/day) improved cardiac dysfunction and increased food intake by 13.5 and 14.2% (both P < 0.01), and rate of weight gain by 95% (P < 0.05) and 1.71-fold (P < 0.01), respectively. The plasma glucose were increased by 49.7 and 50.8% (both P < 0.01), and myocardial glucogen, by 40.5 and 51.7% (both P < 0.01), but blood lactate decreased by 1.56- and 1.96-fold (both P < 0.01), and myocardial lactate by 32.1 and 48.7% (both P < 0.05), respectively. Their MCT1 mRNA and protein expressions increased. The myocardial ghrelin/GHS-R pathway can be upregulated during CHF. The ghrelin can attenuate cardiac dysfunction and energy metabolic disturbance in CHF rats.

摘要

探讨 Ghrelin 对慢性心力衰竭（CHF）期间心肌能量代谢紊乱的影响。给大鼠皮下注射异丙肾上腺素（ISO） 10 天，同时或不给予 Ghrelin 再注射 10 天。酶联免疫吸附试验测定 Ghrelin 浓度。与对照组相比，ISO 处理的大鼠表现出抑制的心脏功能和高 Ghrelin/GHS-R 表达。这些大鼠还表现出食物摄入和体重减少。血浆葡萄糖和心肌糖原水平降低，但血液和心肌中乳酸水平升高，表明心肌代谢紊乱。与单独给予 ISO 的大鼠相比，给予 Ghrelin（20 和 100μg/kg/天）的大鼠改善了心脏功能，使食物摄入量分别增加了 13.5%和 14.2%（均 P < 0.01），体重增加率分别增加了 95%和 1.71 倍（均 P < 0.05）。血浆葡萄糖分别增加了 49.7%和 50.8%（均 P < 0.01），心肌糖原分别增加了 40.5%和 51.7%（均 P < 0.01），而血液乳酸分别减少了 1.56-和 1.96 倍（均 P < 0.01），心肌乳酸分别减少了 32.1%和 48.7%（均 P < 0.05）。它们的 MCT1 mRNA 和蛋白表达增加。CHF 期间，Ghrelin 可以上调心肌 Ghrelin/GHS-R 途径。Ghrelin 可以减轻 CHF 大鼠的心脏功能障碍和能量代谢紊乱。