Xu Zhiwei, Wu Weikang, Lan Taohua, Zhang Xuanhong
Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China.
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(21):2786-9.
To explore the protective effects of extract of Ginkgo biloba (EGB) in adriamycin (ADR)-induced heart failure (HF) and the mechanism of ghrelin peptide.
Wistar rats were randomly divided into three groups: control group, HF group and EGB group. ADR was injected in the rats of HF group and EGB group by caudal vein. After the last injection, the rats in EGB group were given intra-gastric administration of EGB solution (100 mg x kg(-1) x d(-1)). Three weeks later, cardiac function was detected; Ghrelin levels in plasma and myocardium were measured by radio-immunology assay (RIA); High energy phosphates (HEP) contents in myocardium were measured by HPLC; Myocardial gene expression of ghrelin was measured by RT-PCR.
Compared with control group, HF group had obviously decreased index of cardiac function, and these indexes such as +/- dp/dt max in EGB group were higher than those in ADR group. Plasma ghrelin level in HF group was higher than that in control group while myocardial ghrelin level was significantly lower than that in control group. Myocardial ATP content and gene expression of ghrelin mRNA in HF group were significantly lower than those in control group; Plasma ghrelin level in EGB group was significantly increased. Myocardial ATP content and gene expression of ghrelin mRNA in EGB group were significantly higher than those in HF group, and were closed to those of control group.
Myocardial energy dysfunction is an important reason of ADR-induced HF. EGB therapy can improve cardiac function and energy metabolism in HF rats, partly because it might increase the expression and production of ghrelin, which can promote positive energy metabolism.
探讨银杏叶提取物（EGB）对阿霉素（ADR）诱导的心力衰竭（HF）的保护作用及胃饥饿素肽的作用机制。
将Wistar大鼠随机分为三组：对照组、HF组和EGB组。HF组和EGB组大鼠经尾静脉注射ADR。末次注射后，EGB组大鼠给予EGB溶液灌胃（100 mg·kg⁻¹·d⁻¹）。三周后，检测心功能；采用放射免疫分析法（RIA）测定血浆和心肌中胃饥饿素水平；采用高效液相色谱法（HPLC）测定心肌中高能磷酸化合物（HEP）含量；采用逆转录聚合酶链反应（RT-PCR）测定心肌中胃饥饿素的基因表达。
与对照组相比，HF组心功能指标明显降低，EGB组的±dp/dt max等指标高于ADR组。HF组血浆胃饥饿素水平高于对照组，而心肌胃饥饿素水平明显低于对照组。HF组心肌ATP含量和胃饥饿素mRNA基因表达明显低于对照组；EGB组血浆胃饥饿素水平明显升高。EGB组心肌ATP含量和胃饥饿素mRNA基因表达明显高于HF组，且接近对照组。
心肌能量功能障碍是ADR诱导HF的重要原因。EGB治疗可改善HF大鼠的心功能和能量代谢，部分原因可能是其增加了胃饥饿素的表达和产生，从而促进了正能量代谢。
