与 DNase I 和 II 相比，人工核酸酶“Mn-TMPyP/KHSO5”诱导 DNA 产生的末端磷酸的 31P NMR 表征。
31P NMR characterization of terminal phosphates induced on DNA by the artificial nuclease 'Mn-TMPyP/KHSO5' in comparison with DNases I and II.
作者信息
Gasmi G, Pasdeloup M, Pratviel G, Pitié M, Bernadou J, Meunier B
机构信息
Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
出版信息
Nucleic Acids Res. 1991 Jun 11;19(11):2835-9. doi: 10.1093/nar/19.11.2835.
Abstract
Phosphorus-31 NMR has been applied to the characterization of terminal phosphates on fragments of calf thymus DNA induced by three different nuclease systems: DNase I, DNase II and the artificial nuclease 'Mn-TMPyP/KHSO5'. In this last case, the oxidative damage to deoxyribose leads to two monophosphates esters (at the 3' and 5' ends) on both sides of the cleavage site. This method constitutes a promising approach to visualise the phosphate termini generated in DNA or RNA cleavage by cytotoxic drugs or chemical nucleases and provides a novel insight into the molecular aspects of their mechanism of action.
摘要
磷-31核磁共振已被用于表征由三种不同核酸酶系统诱导的小牛胸腺DNA片段上的末端磷酸盐：脱氧核糖核酸酶I、脱氧核糖核酸酶II和人工核酸酶“锰-四甲基吡啶卟啉/过硫酸氢钾”。在最后一种情况下，对脱氧核糖的氧化损伤会在切割位点两侧产生两种单磷酸酯（在3'和5'末端）。该方法为可视化细胞毒性药物或化学核酸酶切割DNA或RNA时产生的磷酸盐末端提供了一种有前景的方法，并为其作用机制的分子层面提供了新的见解。
相似文献
1
31P NMR characterization of terminal phosphates induced on DNA by the artificial nuclease 'Mn-TMPyP/KHSO5' in comparison with DNases I and II.与 DNase I 和 II 相比，人工核酸酶“Mn-TMPyP/KHSO5”诱导 DNA 产生的末端磷酸的 31P NMR 表征。
Nucleic Acids Res. 1991 Jun 11;19(11):2835-9. doi: 10.1093/nar/19.11.2835.
2
Use of phosphorus ligand NMR probes to investigate electronic and second-sphere solvent effects in ligand substitution reactions at manganese(II) and manganese(III).使用磷配体核磁共振探针研究锰（II）和锰（III）配体取代反应中的电子效应和第二配位层溶剂效应。
Inorg Chem. 2005 May 16;44(10):3405-11. doi: 10.1021/ic048618n.
3
Theoretical study of the interaction between a high-valent manganese porphyrin oxyl-(hydroxo)-Mn(IV)-TMPyP and double-stranded DNA.高价锰卟啉氧基 -（羟基）-Mn(IV)-TMPyP与双链DNA相互作用的理论研究
J Comput Chem. 2003 May;24(7):797-805. doi: 10.1002/jcc.10212.
4
A single nuclease-resistant linkage in DNA as a versatile tool for the characterization of DNA lesions: application to the guanine oxidative lesion "G+34" generated by metalloporphyrin/KHSO(5) reagent.DNA 中单链核酸酶抗性连接作为一种用于 DNA 损伤特征分析的多功能工具：应用于金属卟啉/KHSO(5)试剂产生的鸟嘌呤氧化损伤“G+34”。
Chem Res Toxicol. 2012 Nov 19;25(11):2505-12. doi: 10.1021/tx300319y. Epub 2012 Oct 12.
5
Potassium monopersulfate and a water-soluble manganese porphyrin complex, [Mn(TMPyP)](OAc)5, as an efficient reagent for the oxidative cleavage of DNA.
Biochemistry. 1989 Sep 5;28(18):7268-75. doi: 10.1021/bi00444a019.
6
Interaction of cationic nickel and manganese porphyrins with the minor groove of DNA.阳离子镍和锰卟啉与 DNA 小沟的相互作用。
Inorg Chem. 2010 Sep 20;49(18):8558-67. doi: 10.1021/ic101178n.
7
Oxidative cleavage of DNA mediated by hybrid metalloporphyrin-ellipticine molecules and functionalized metalloporphyrin precursors.由杂化金属卟啉-玫瑰树碱分子和功能化金属卟啉前体介导的DNA氧化裂解
Biochemistry. 1990 Aug 28;29(34):7868-75. doi: 10.1021/bi00486a013.
8
[Sequence-dependent cleavage of HBV DNA by combining with triple helix-forming oligodeoxyribonucleotides modified with manganese porphyrin in vitro].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Sep;19(3):282-5.
9
Preferential hydroxylation by the chemical nuclease meso-tetrakis-(4-N-methylpyridiniumyl)porphyrinatomanganeseIII pentaacetate/KHSO5 at the 5' carbon of deoxyriboses on both 3' sides of three contiguous A.T base pairs in short double-stranded oligonucleotides.化学核酸酶中-四（4-N-甲基吡啶基）卟啉锰III五乙酸酯/KHSO5对短双链寡核苷酸中三个相邻A.T碱基对两侧3'端脱氧核糖5'碳进行优先羟基化作用。
Proc Natl Acad Sci U S A. 1992 May 1;89(9):3967-71. doi: 10.1073/pnas.89.9.3967.
10
Structure/nuclease activity relationships of DNA cleavers based on cationic metalloporphyrin-oligonucleotide conjugates.基于阳离子金属卟啉-寡核苷酸共轭物的DNA切割剂的结构/核酸酶活性关系
Biochemistry. 1996 Jul 16;35(28):9140-9. doi: 10.1021/bi9530402.
引用本文的文献
1
2
Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA.使用分子信标作为单链DNA酶切的灵敏荧光检测方法。
Nucleic Acids Res. 2000 Jun 1;28(11):E52. doi: 10.1093/nar/28.11.e52.
3
Mechanisms of DNA damage by chromium(V) carcinogens.铬（V）致癌物导致DNA损伤的机制。
Nucleic Acids Res. 1998 Apr 1;26(7):1588-96. doi: 10.1093/nar/26.7.1588.
4
Preferential hydroxylation by the chemical nuclease meso-tetrakis-(4-N-methylpyridiniumyl)porphyrinatomanganeseIII pentaacetate/KHSO5 at the 5' carbon of deoxyriboses on both 3' sides of three contiguous A.T base pairs in short double-stranded oligonucleotides.化学核酸酶中-四（4-N-甲基吡啶基）卟啉锰III五乙酸酯/KHSO5对短双链寡核苷酸中三个相邻A.T碱基对两侧3'端脱氧核糖5'碳进行优先羟基化作用。
Proc Natl Acad Sci U S A. 1992 May 1;89(9):3967-71. doi: 10.1073/pnas.89.9.3967.
本文引用的文献
1
DNA strand scission activity of metalloporphyrins.金属卟啉的DNA链断裂活性
Biochem Biophys Res Commun. 1982 Aug;107(3):1067-74. doi: 10.1016/0006-291x(82)90630-1.
2
Hemin-mediated DNA strand scission.血红素介导的DNA链断裂。
J Biol Chem. 1983 Oct 10;258(19):12069-72.
3
Cleavage of DNA with methidiumpropyl-EDTA-iron(II): reaction conditions and product analyses.用甲啶丙基-乙二胺四乙酸-铁（II）切割DNA：反应条件及产物分析
Biochemistry. 1984 Aug 14;23(17):3934-45. doi: 10.1021/bi00312a022.
4
Porphyrin localization: a new modality for detection and therapy of tumors.
Biochem Pharmacol. 1984 May 1;33(9):1389-93. doi: 10.1016/0006-2952(84)90403-9.
5
DNA cleavage specificity of a group of cationic metalloporphyrins.一组阳离子金属卟啉的DNA切割特异性
Biochemistry. 1986 Nov 4;25(22):6875-83. doi: 10.1021/bi00370a021.
6
Synthesis and characterization of oligodeoxyribonucleotides containing terminal phosphates. NMR, UV spectroscopic and thermodynamic analysis of duplex formation of [d(pGGAATTCC)]2, [d(GGAATTCCp)]2 and [d(pGGAATTCCp)]2.含末端磷酸的寡脱氧核糖核苷酸的合成与表征。[d(pGGAATTCC)]2、[d(GGAATTCCp)]2和[d(pGGAATTCCp)]2双链体形成的核磁共振、紫外光谱和热力学分析。
Nucleic Acids Res. 1987 Apr 24;15(8):3531-47. doi: 10.1093/nar/15.8.3531.
7
Photocleavage of DNA in the presence of synthetic water-soluble porphyrins.在合成水溶性卟啉存在下DNA的光裂解作用
Photochem Photobiol. 1986 Dec;44(6):717-24. doi: 10.1111/j.1751-1097.1986.tb05529.x.
8
Metals and DNA: molecular left-handed complements.金属与DNA：分子左旋互补物
Science. 1986 Aug 15;233(4765):727-34. doi: 10.1126/science.3016894.
9
Porphyrin photosensitization and phototherapy.
Photochem Photobiol. 1986 Jun;43(6):681-90. doi: 10.1111/j.1751-1097.1986.tb05647.x.
10
Hydroxyl radical footprinting: a high-resolution method for mapping protein-DNA contacts.羟基自由基足迹法：一种用于绘制蛋白质 - DNA 相互作用图谱的高分辨率方法。
Methods Enzymol. 1987;155:537-58. doi: 10.1016/0076-6879(87)55035-2.
Zotero 插件