Gasmi G, Pasdeloup M, Pratviel G, Pitié M, Bernadou J, Meunier B
Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
Nucleic Acids Res. 1991 Jun 11;19(11):2835-9. doi: 10.1093/nar/19.11.2835.
Phosphorus-31 NMR has been applied to the characterization of terminal phosphates on fragments of calf thymus DNA induced by three different nuclease systems: DNase I, DNase II and the artificial nuclease 'Mn-TMPyP/KHSO5'. In this last case, the oxidative damage to deoxyribose leads to two monophosphates esters (at the 3' and 5' ends) on both sides of the cleavage site. This method constitutes a promising approach to visualise the phosphate termini generated in DNA or RNA cleavage by cytotoxic drugs or chemical nucleases and provides a novel insight into the molecular aspects of their mechanism of action.
磷-31核磁共振已被用于表征由三种不同核酸酶系统诱导的小牛胸腺DNA片段上的末端磷酸盐:脱氧核糖核酸酶I、脱氧核糖核酸酶II和人工核酸酶“锰-四甲基吡啶卟啉/过硫酸氢钾”。在最后一种情况下,对脱氧核糖的氧化损伤会在切割位点两侧产生两种单磷酸酯(在3'和5'末端)。该方法为可视化细胞毒性药物或化学核酸酶切割DNA或RNA时产生的磷酸盐末端提供了一种有前景的方法,并为其作用机制的分子层面提供了新的见解。