National Allergy Research Centre, Department of Dermatology and Allergy Centre, Odense University Hospital, Odense, Denmark.
Acta Derm Venereol. 2010 Jul;90(4):374-8. doi: 10.2340/00015555-0874.
Vesicular systems, such as liposomes and ethosomes, are used in cosmetic and pharmaceutical products to encapsulate ingredients, to protect ingredients from degradation, to increase bioavailability, and to improve cosmetic performance. Some reports have suggested that formulation of cosmetic ingredients in vesicular carrier systems may increase their contact allergy elicitation potential in humans. However, no sensitization studies have been published. We formulated two model contact allergens (isoeugenol and dinitrochlorobenzene) in ethosomes and investigated the sensitization response using a modified local lymph node assay (LLNA). The results were compared with those for the same allergens in similar concentrations and vehicles without ethosomes. Both allergens encapsulated in 200-300 nm ethosomes showed increased sensitizing potency in the murine assay compared with the allergens in solution without ethosomes. Empty ethosomes were non-sensitizing according to LLNA. The clinical implications are so far uncertain, but increased allergenicity from ethosome-encapsulated topical product ingredients cannot be excluded.
囊泡系统，如脂质体和醇质体，被用于化妆品和药物产品中，以包裹成分、保护成分免受降解、增加生物利用度并改善化妆品性能。一些报告表明，将化妆品成分制成囊泡载体系统可能会增加其在人类中引发接触过敏的潜力。然而，目前还没有发表关于致敏性的研究。我们将两种模型接触过敏原（异丁香酚和二硝基氯苯）制成醇质体，并使用改良的局部淋巴结检测（LLNA）来研究致敏反应。将结果与相同浓度和不含醇质体的相同载体中的相同过敏原进行比较。与不含醇质体的溶液中的过敏原相比，包裹在 200-300nm 醇质体中的两种过敏原在小鼠检测中表现出更高的致敏效力。根据 LLNA，空醇质体不具有致敏性。目前，其临床意义尚不确定，但不能排除含有醇质体的局部产品成分的变应原性增加。
