Cheng De, Lei Lei, Lu Zhijuan, Li Zhen, Wang Huayan
Shaanxi Key Laboratory of Agricultural Molecular Biology, Shaanxi Center for Stem Cell Engineering and Technology, College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China.
Sheng Wu Gong Cheng Xue Bao. 2010 Apr;26(4):421-30.
The somatic cells can be induced into ES-like stem cells when retrovirally infected the defined transcription factors including Oct4, Sox2, Klf4 and c-Myc. These ES-like cells are named induced pluripotent stem (iPS) cells and this method is called iPS technology. Until the end of 2009, iPS cell lines have been generated in various animal species, such as mouse, human, rhesus monkey, rat and pig. Mouse iPS cells are also used to generate chimera mice and viable mice through the tetraploid complementation. Although iPS cells are extremely similar to ES cells in both morphology and growth features, to generate iPS cells do need the defined culture procedures. Based on the update global iPS technology development and the iPS studies in our laboratory, this paper focused on the establishment of iPS cell lines and improvement of iPS cell culture condition.
当用逆转录病毒感染包括Oct4、Sox2、Klf4和c-Myc在内的特定转录因子时，体细胞可被诱导成为类胚胎干细胞。这些类胚胎干细胞被称为诱导多能干细胞（iPS细胞），此方法被称为iPS技术。截至2009年底，已在多种动物物种中产生了iPS细胞系，如小鼠、人类、恒河猴、大鼠和猪。小鼠iPS细胞也被用于通过四倍体互补产生嵌合体小鼠和存活小鼠。尽管iPS细胞在形态和生长特征上与胚胎干细胞极为相似，但生成iPS细胞确实需要特定的培养程序。基于全球iPS技术的最新发展以及我们实验室的iPS研究，本文重点关注iPS细胞系的建立和iPS细胞培养条件的改进。
