He Fan, Sun Yuan, Ge Jinying, Li Miao, Chang Tianming, Bu Zhigao, Qiu Huaji
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Sheng Wu Gong Cheng Xue Bao. 2010 Apr;26(4):439-47.
In order to ensure the biosafety of the IFN-gamma antiviral activity assay, we used a replication-deficient VSV carrying GFP as an interferon sensitive indicator virus (VSVdeltaGG). The antiviral activities of porcine IFN-gamma expressed in Escherichia coli and in baculovirus on MDBK cells were assessed. The results showed that the antiviral activity of porcine IFN-gamma expressed in baculovirus could reach 10(5) IU/mL, while the porcine IFN-gamma expressed in E. coli showed some antiviral activity (32 IU/mL) after refolding. The results of the VSVdeltaGG-based antiviral assay were almost identical to that of the VSVGFP-based assay, suggesting it is highly feasible to use VSVdeltaGG as a substitute for VSV*GFP, making assays for IFN-gamma antiviral activity safer and more accurate.
为确保干扰素γ抗病毒活性测定的生物安全性，我们使用了一种携带绿色荧光蛋白（GFP）的复制缺陷型水疱性口炎病毒（VSV）作为干扰素敏感指示病毒（VSVdeltaGG）。评估了在大肠杆菌和杆状病毒中表达的猪干扰素γ对MDBK细胞的抗病毒活性。结果表明，杆状病毒表达的猪干扰素γ的抗病毒活性可达10(5) IU/mL，而大肠杆菌表达的猪干扰素γ在复性后显示出一定的抗病毒活性（32 IU/mL）。基于VSVdeltaGG的抗病毒测定结果与基于VSVGFP的测定结果几乎相同，表明使用VSVdeltaGG替代VSV*GFP具有高度可行性，使得干扰素γ抗病毒活性测定更安全、更准确。
