School of Chemistry, University of Southampton, Southampton, SO17 1BJ, UK.
Phys Chem Chem Phys. 2010 Sep 14;12(34):10018-26. doi: 10.1039/c0cp00305k. Epub 2010 Jun 24.
Surface modification techniques are essential to the construction of enzyme based elements of biofuel cells and biosensors. In this article we report on the preparation and characterisation of modified carbon electrodes which were used as supports for the immobilisation of laccase from Trametes hirsuta. The electrodes were electrochemically modified with diamine or diazonium linkers followed by attachment of either anthracene or anthraquinone head groups using solid phase chemical methodology. These well defined surfaces were found to effectively bind laccase and to provide direct electrical contact to the enzyme active site, as evidenced by XPS, EIS and voltammetry, respectively. The influence of the type of linker and head group on enzyme binding and bioelectrocatalytic activity are evaluated.
表面修饰技术对于构建基于酶的生物燃料电池和生物传感器元件至关重要。本文报道了经过修饰的碳电极的制备和表征，这些电极被用作固定化来自糙皮侧耳木霉的漆酶的载体。电极通过二胺或重氮键合试剂进行电化学修饰，然后使用固相化学方法将蒽或蒽醌基团连接到电极上。这些高度明确的表面被发现可以有效地结合漆酶，并通过 XPS、EIS 和伏安法分别提供与酶活性位点的直接电接触。评价了链接剂和头基的类型对酶结合和生物电化学催化活性的影响。
