Department of Chemical and Environmental Technology, ESCET, Universidad Rey Juan Carlos. C/ Tulipán s/n, Móstoles, Madrid, Spain.
Bioresour Technol. 2010 Nov;101(22):8541-8. doi: 10.1016/j.biortech.2010.06.042.
The oxidation of naphthalene by immobilized laccase from Trametes versicolor has been performed using diverse immobilization strategies on mesostructured silica materials. Laccase was immobilized by physical adsorption on several SBA-15 with different textural properties and by covalent attachment on functionalized SBA-15 prepared by co-condensation method (direct synthesis). The adsorption of laccase was partially reversible and showed some degree of lixiviation. However, covalently attached laccase to aminopropyl and aminobutyl functionalized SBA-15 exhibited important activity for the degradation of naphthalene with, respectively, 35% and 39%wt of removal in 5h. The aminopropyl biocatalyst retained higher activity after repeated uses than the corresponding aminobutyl.
固定化漆酶对苊的氧化作用采用不同的介孔硅材料固定化策略进行。通过物理吸附将漆酶固定在具有不同结构特性的几种 SBA-15 上，通过共缩合方法（直接合成）制备的功能化 SBA-15 进行共价连接。漆酶的吸附部分是可逆的，并且表现出一定程度的浸析。然而，共价连接到氨基丙基和氨基丁基功能化 SBA-15 的漆酶在 5 小时内分别具有 35%和 39%wt 的苊去除率，表现出对苊降解的重要活性。氨基丙基生物催化剂在重复使用后保持了比相应的氨基丁基更高的活性。
