In vivo visualization of trophozoites in patients with amoebic colitis by using a newly developed endocytoscope.

BACKGROUND

The endocytoscopy system (ECS) is a new method to provide real-time super-magnifying microscopic imaging in vivo. Routine diagnosis of amebic colitis requires multiple tests that are both time consuming and costly.

OBJECTIVE

To determine the feasibility of ECS to directly detect the amebic parasites in vivo.

DESIGN

Prospective, single-center, pilot study.

SETTING

Tertiary-care university hospital.

PATIENTS

This study involved 5 patients who were suspected to have amebic colitis by conventional colonoscopy in our institute.

INTERVENTIONS

A super-magnifying ECS with 450 x magnification.

MAIN OUTCOME MEASUREMENTS

We compared ECS findings with those of conventional methods-serum antibody tests and histology of colon biopsy specimens.

RESULTS

We successfully visualized the amebic trophozoites in all 5 cases. In contrast, 3 specimens had positive results on serology, and 3 had positive histology results on hematoxylin and eosin staining.

LIMITATIONS

Pilot study with a limited number of patients. Findings were compared only with serology and histology findings.

CONCLUSIONS

ECS would be a useful tool for the prompt diagnosis of amebic colitis via the real-time in vivo visualization of amebic trophozoites.