Université de Limoges, Groupement de Recherche Eau Sol Environnement, EA 4330, 123 Av Albert Thomas, 87 060 Limoges, France.
Colloids Surf B Biointerfaces. 2010 Oct 15;80(2):161-8. doi: 10.1016/j.colsurfb.2010.05.043. Epub 2010 Jun 4.
The effects of the extraction procedure of extracellular polymeric substances (EPS) on their proton/metal binding properties were studied. Nine extraction procedures (one control, four physical and four chemical procedures) were applied to four types of anaerobic granular sludges. The binding capacities between the EPS and lead or cadmium were investigated at pH 7 by a polarographic method. The composition of the EPS extracts varied according to the extraction technique and the origin of the sludge. This induced differences in the pK(a)s and the binding sites density of the EPS extracts. The carry-over of the extractant in the samples strongly affects the properties of the EPS from chemical extraction protocols. Lead and cadmium seem to be bound differently with the EPS, a higher binding capacity was observed for Pb(2+) than for Cd(2+).
研究了提取细胞外聚合物（EPS）的方法对其质子/金属结合特性的影响。采用九种提取方法（一种对照，四种物理方法和四种化学方法）对四种厌氧颗粒污泥进行处理。通过极谱法在 pH 7 下研究了 EPS 与铅或镉之间的结合能力。根据提取技术和污泥的来源，EPS 提取物的组成有所不同。这导致 EPS 提取物的 pK(a)和结合位点密度存在差异。在样品中携带的萃取剂强烈影响化学提取方案中 EPS 的性质。铅和镉似乎与 EPS 不同程度地结合，观察到 Pb(2+)的结合能力高于 Cd(2+)。
