剪接体组装因子 SC-35 在二尖瓣和三尖瓣反流中 TUNEL 阳性心房肌细胞中的表达：心房肌细胞的活力。

Expression of spliceosome assembly factor SC-35 in TUNEL-positive atrial cardiomyocytes in mitral and tricuspid regurgitation: viability of atrial cardiomyocytes.

机构信息

Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Taiwan, ROC.

出版信息

Int J Cardiol. 2011 Sep 15;151(3):323-7. doi: 10.1016/j.ijcard.2010.06.006. Epub 2010 Jun 26.

DOI:10.1016/j.ijcard.2010.06.006

PMID:20580447

Abstract

BACKGROUND

Most of the atrial cardiomyocytes with positive terminal deoxynucleotidyl transferase (TdT)-mediated dUTP in situ nick end-labelling (TUNEL) reaction are not apoptotic in patients with mitral and tricuspid valve diseases. The TUNEL-positive myocytes with expression of spliceosome assembly factor SC-35, an indicator of increased RNA synthesis, should be living cardiomyocytes.

METHODS

This study analyzed twenty-three patients with significant mitral and tricuspid regurgitation. Fifteen patients had persistent atrial fibrillation, and eight had sinus rhythm. Atrial appendageal tissues were obtained during surgery. Immunohistochemical study was performed.

RESULTS

Immunohistochemical study of fibrillating right atrial myocardium demonstrated that 44.8 ± 24.6% of myocytes had TUNEL-positive nuclei whereas 39.4 ± 21.4% of myocytes had TUNEL-positive nuclei in sinus right atrial myocardium (p=0.682). However, most (81.6%) nuclei of TUNEL-positive myocytes in the fibrillating right atria also expressed proliferating cell nuclear antigen (PCNA), an indicator of DNA replication and repair, and most nuclei (91.8%) of TUNEL-positive myocytes also expressed SC-35. In fibrillating left atria, most (88.1%) nuclei of TUNEL-positive myocytes also expressed SC-35. Similarly, in sinus right atrial myocardium, most (78.0%) nuclei of TUNEL-positive myocytes expressed PCNA, and most (91.4%) nuclei of TUNEL-positive myocytes also expressed SC-35, but none expressed Ki-67, a replication-associated antigen. Additionally, the percentage of TUNEL-positive myocytes in the right atria significantly and positively correlated with the percentage of PCNA-positive myocytes (r=0.826, p<0.001) and SC-35 positive myocytes (r=0.713, p<0.001).

CONCLUSIONS

Most TUNEL-positive atrial cardiomyocytes in patients with mitral and tricuspid regurgitation are living cardiomyocytes.

摘要

背景

在患有二尖瓣和三尖瓣疾病的患者中，大多数具有阳性末端脱氧核苷酸转移酶（TdT）介导的 dUTP 原位切口末端标记（TUNEL）反应的心房肌细胞不是凋亡的。表达剪接体组装因子 SC-35 的 TUNEL 阳性肌细胞，是 RNA 合成增加的指标，应该是存活的心肌细胞。

方法

本研究分析了 23 例有明显二尖瓣和三尖瓣反流的患者。15 例患者持续性心房颤动，8 例窦性节律。手术期间获取心耳组织。进行免疫组织化学研究。

结果

右心房颤动心肌的免疫组织化学研究表明，44.8%±24.6%的肌细胞核呈 TUNEL 阳性，而窦性右心房心肌的 TUNEL 阳性肌细胞核为 39.4%±21.4%（p=0.682）。然而，在颤动的右心房中，大多数（81.6%）TUNEL 阳性肌细胞核也表达增殖细胞核抗原（PCNA），这是 DNA 复制和修复的指标，大多数 TUNEL 阳性肌细胞核也表达 SC-35（91.8%）。在颤动的左心房中，大多数（88.1%）TUNEL 阳性肌细胞核也表达 SC-35。同样，在窦性右心房心肌中，大多数（78.0%）TUNEL 阳性肌细胞核表达 PCNA，大多数（91.4%）TUNEL 阳性肌细胞核也表达 SC-35，但均不表达 Ki-67，这是一种与复制相关的抗原。此外，右心房 TUNEL 阳性肌细胞的百分比与 PCNA 阳性肌细胞（r=0.826，p<0.001）和 SC-35 阳性肌细胞（r=0.713，p<0.001）的百分比呈显著正相关。