Department of Cellular and Molecular Physiopathology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
Neuroscience. 2010 Sep 1;169(3):1473-85. doi: 10.1016/j.neuroscience.2010.04.058. Epub 2010 May 24.
Two monoclonal antibodies (Nilo1 and Nilo2) were generated after immunization of hamsters with E13.5 olfactory bulb-derived mouse neurospheres. They are highly specific for neural stem and early progenitor cell surface antigens. Nilo positive cells present in the adult mouse subventricular zone (SVZ) were able to initiate primary neural stem cell cultures. Moreover, these antibodies added to neurosphere cultures induced proliferation arrest and interfered with their differentiation. In the lateral ventricles of adult mice, Nilo1 stained a cell subpopulation lining the ventricle and cells located in the SVZ, whereas Nilo2 stained a small population associated with the anterior horn of the SVZ at the beginning of the rostral migratory stream. Co-staining of Nilo1 or Nilo2 and neural markers demonstrated that Nilo1 identifies an early neural precursor subpopulation, whereas Nilo2 detects more differentiated neural progenitors. Thus, these antibodies identify distinct neurogenic populations within the SVZ of the lateral ventricle.
两种单克隆抗体（Nilo1 和 Nilo2）是通过用 E13.5 嗅球来源的小鼠神经球免疫仓鼠而产生的。它们高度特异性地针对神经干细胞和早期祖细胞表面抗原。成年小鼠侧脑室（SVZ）中的 Nilo 阳性细胞能够启动原发性神经干细胞培养。此外，这些抗体添加到神经球培养物中会诱导增殖停滞并干扰其分化。在成年小鼠的侧脑室中，Nilo1 染色了位于脑室衬里的细胞亚群和位于 SVZ 中的细胞，而 Nilo2 染色了与 SVZ 前角相关的一小部分细胞，位于嗅流的起始处。Nilo1 或 Nilo2 与神经标记物的共染色表明，Nilo1 鉴定了早期神经前体细胞亚群，而 Nilo2 检测到分化程度更高的神经祖细胞。因此，这些抗体在侧脑室的 SVZ 中鉴定出不同的神经发生群体。
