利用基于荧光共振能量转移的活性生物传感器可视化激酶活性。
Visualization of kinase activity with FRET-based activity biosensors.
作者信息
Depry Charlene, Zhang Jin
机构信息
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
出版信息
Curr Protoc Mol Biol. 2010 Jul;Chapter 18:Unit 18.15. doi: 10.1002/0471142727.mb1815s91.
PMID:20583095
Abstract
Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy.
摘要
基于基因编码荧光共振能量转移的激酶活性报告分子（KARs）能够在活细胞中以高时空分辨率实时监测激酶活性动态变化。本单元描述了一种利用KARs通过荧光显微镜观察活的哺乳动物细胞中激酶活性的通用方案。
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