Nakata H, Shimizu A, Tajima A, Lin S Z, Gruber K, Perillo E, Peress N, Fenstermacher J
Department of Neurological Surgery, State University of New York, Stony Brook 11794.
Acta Neuropathol. 1991;81(3):312-7. doi: 10.1007/BF00305873.
The level of cerebrospinal fluid (CSF) protein is elevated in diseases and disease models that are associated with circulating immune complexes such as serum sickness. Circulatory immune complexes are known to deposit in the basal lamina of fenestrated capillaries and may, as a result, affect both capillary bed and parenchymal function. Since the brain has both fenestrated and unfenestrated capillaries and immune complexes deposit to a varying extent in the fenestrated capillaries in chronic serum sickness, cerebral capillary permeability to protein may be altered in some brain areas and lead to the elevation of CSF proteins. In addition various other cerebrovascular and metabolic functions may also be affected by this condition. In this study either radio-iodinated serum albumin (RISA) or 2-[14C]deoxyglucose (14C-2DG) was intravenously injected into control Wistar rats and Wistar rats with chronic serum sickness; subsequently the tissue levels of radioactivity were measured by quantitative autoradiography in 4 brain areas with fenestrated capillaries and 11 brain areas with unfenestrated capillaries. The 2-min distribution of RISA, which demarcates the volume of circulating plasma in perfused microvessels and is generally proportional to local plasma flow, was the same in control and experimental rats. The passage of RISA from blood into brain over 30 min was negligible in both groups; thus cerebral capillary permeability to albumin was not detectably increased in any of these 15 brain areas by chronic serum sickness. The rate of local cerebral glucose utilization, an indicator of local metabolic and neural activity, was calculated from the 14C-2DG data and was virtually identical in control and experimental rats.(ABSTRACT TRUNCATED AT 250 WORDS)
在与循环免疫复合物相关的疾病和疾病模型（如血清病）中，脑脊液（CSF）蛋白水平会升高。已知循环免疫复合物会沉积在有窗孔毛细血管的基底层，因此可能会影响毛细血管床和实质功能。由于大脑既有有窗孔的毛细血管，也有无窗孔的毛细血管，且在慢性血清病中免疫复合物在有窗孔的毛细血管中有不同程度的沉积，所以在某些脑区，脑毛细血管对蛋白质的通透性可能会改变，导致脑脊液蛋白升高。此外，这种情况还可能影响各种其他脑血管和代谢功能。在本研究中，将放射性碘标记的血清白蛋白（RISA）或2-[¹⁴C]脱氧葡萄糖（¹⁴C-2DG）静脉注射到对照Wistar大鼠和患有慢性血清病的Wistar大鼠体内；随后，通过定量放射自显影术测量了4个有窗孔毛细血管的脑区和11个无窗孔毛细血管的脑区的放射性组织水平。RISA的2分钟分布界定了灌注微血管中循环血浆的体积，通常与局部血浆流量成正比，在对照大鼠和实验大鼠中是相同的。两组中，30分钟内RISA从血液进入大脑的量都可以忽略不计；因此，慢性血清病在这15个脑区中的任何一个都未检测到脑毛细血管对白蛋白的通透性明显增加。根据¹⁴C-2DG数据计算局部脑葡萄糖利用率，这是局部代谢和神经活动的指标，在对照大鼠和实验大鼠中几乎相同。（摘要截短于250字）
