3rd Department of Pediatrics, Aristotle University, Thessaloniki, Greece.
Antimicrob Agents Chemother. 2010 Sep;54(9):3985-7. doi: 10.1128/AAC.01799-09. Epub 2010 Jun 28.
Using a liquid chromatography-tandem mass spectrometry method, the serum and cerebrospinal fluid (CSF) concentrations of colistin were determined in patients aged 1 months to 14 years receiving intravenous colistimethate sodium (60,000 to 225,000 IU/kg of body weight/day). Only in one of five courses studied (a 14-year-old receiving 225,000 IU/kg/day) did serum concentrations exceed the 2 microg/ml CLSI/EUCAST breakpoint defining susceptibility to colistin for Pseudomonas and Acinetobacter. CSF colistin concentrations were <0.2 microg/ml but increased in the presence of meningitis (approximately 0.5 microg/ml or 34 to 67% of serum levels).
采用液相色谱-串联质谱法,测定了 1 个月至 14 岁接受静脉注射黏菌素甲磺酸钠(60000 至 225000 IU/kg/天)治疗的患者的血清和脑脊液(CSF)中黏菌素浓度。在研究的五个疗程中只有一个(一个 14 岁的患者接受 225000 IU/kg/天)的血清浓度超过了 2 μg/ml CLSI/EUCAST 折点,该折点定义了黏菌素对铜绿假单胞菌和不动杆菌的敏感性。CSF 黏菌素浓度<0.2μg/ml,但在存在脑膜炎时增加(约 0.5μg/ml 或血清水平的 34 至 67%)。