Minina Iu M, Zhdanova N S, Shilov A G, Tolkunova E N, Liskovykh M A, Tomilin A N
Tsitologiia. 2010;52(5):420-5.
A perspective of using embryonic stem (ES) and induced pluripotent stem (iPS) cells in clinical medicine makes karyological analysis of these cells an important issue. Using methods of classical and molecular cytogenetics chromosomal analysis, we have carried out karyological study of two mouse ES and two iPS cell lines derived de novo. We have found monosomy of X chromosome in all studied ES and iPS cell lines, thus making a modal number of chromosomes in these cell lines 39. A chromosomal instability (aneuploidy) was revealed in both studied iPS cell lines. Moreover, we have detected chromosomal rearrangements and chromosomal fragments in one of iPS cell line. Our findings underline the importance of careful cytogenetic evaluation of pluripotent cell lines, especially iPS cell lines, which should be carried out prior to any clinical use of these cells.
在临床医学中使用胚胎干细胞（ES）和诱导多能干细胞（iPS）的前景使得对这些细胞进行染色体分析成为一个重要问题。我们运用经典和分子细胞遗传学染色体分析方法，对新建立的两个小鼠ES细胞系和两个iPS细胞系进行了染色体研究。我们发现，在所有研究的ES和iPS细胞系中均存在X染色体单体，因此这些细胞系的染色体众数为39。在两个研究的iPS细胞系中均发现了染色体不稳定性（非整倍体）。此外，我们在其中一个iPS细胞系中检测到了染色体重排和染色体片段。我们的研究结果强调了在对多能细胞系，尤其是iPS细胞系进行仔细的细胞遗传学评估的重要性，这应该在这些细胞的任何临床应用之前进行。
