Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Cell Transplant. 2010;19(6):901-9. doi: 10.3727/096368910X509031. Epub 2010 Jun 29.
Induced pluripotent stem (iPS) cells have recently been generated by Yamanaka's group, and then followed by others. iPS cells are expected to have clinical applications including an important role in regenerative medicine. This study focused on the cell-penetrating peptides (CPPs) for differentiation or functional application of iPS cells, because several transduction domains can deliver a large size-independent variety of molecules into cells. Two CPPs, Texas Red-R8 and Rhodamine-TAT, were generated as representative CPPs and these CPPs were tested to determine their ability to penetrate the membrane of iPS cells. Both CPPs were transduced in iPS cells through macropinocytosis classified in endocytosis within 2 h in a manner consistent with many other cells, and no cytotoxicity and influence on their undifferentiated state was observed. In conclusion, CPPs can be utilized for their differentiation or functional application in iPS cells.
诱导多能干细胞（iPS 细胞）最近由山中伸弥团队产生，随后其他团队也相继产生。iPS 细胞有望在再生医学中发挥重要作用，具有临床应用价值。本研究重点关注细胞穿透肽（CPP）在 iPS 细胞的分化或功能应用，因为几个转导域可以将各种大小独立的分子递送到细胞内。生成了两种 CPP（Texas Red-R8 和 Rhodamine-TAT）作为代表性 CPP，并测试了它们穿透 iPS 细胞膜的能力。这两种 CPP 都通过巨胞饮作用在 2 小时内转导到 iPS 细胞中，这种方式与许多其他细胞一致，并且没有观察到细胞毒性和对其未分化状态的影响。总之，CPP 可用于 iPS 细胞的分化或功能应用。
