Siachoque N, Jewtuchowicz V M, Iovannitti C, Mujica M T
Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Rev Argent Microbiol. 2010 Apr-Jun;42(2):91-4. doi: 10.1590/S0325-75412010000200004.
Cryptococcus is an encapsulated yeast of class Basidiomycetes, etiologic agent of cryptococcosis. Cryptococcosis is one of the most common opportunistic infections in immunosuppressed patients, although it can affect immunocompetent individuals. In recent years, the identification of medically important fungal species has been achieved through the amplification of specific regions or genes of fungal DNA by polymerase chain reaction (PCR). The CAP59 gene is involved in the synthesis of the capsule in Cryptococcus neoformans and Cryptococcus gattii, and is useful in the molecular identification of serotypes. In this research, we use yeasts of different serotypes from collection strains and C. neoformans isolates recovered from patients with cryptococcosis. A standardized yeast suspension from different Cryptococcus isolates as template allowed CAP59 gene amplification. This procedure was quick, simple, and inexpensive and required no PCR steps. This is important for taxonomic studies in laboratories with implemented molecular biology tools.
隐球菌是担子菌纲的一种有荚膜酵母菌，是隐球菌病的病原体。隐球菌病是免疫抑制患者中最常见的机会性感染之一，尽管它也可影响免疫功能正常的个体。近年来，通过聚合酶链反应（PCR）扩增真菌DNA的特定区域或基因，已实现了对具有医学重要性的真菌种类的鉴定。CAP59基因参与新型隐球菌和格特隐球菌荚膜的合成，可用于血清型的分子鉴定。在本研究中，我们使用了来自保藏菌株的不同血清型酵母以及从隐球菌病患者中分离出的新型隐球菌菌株。以来自不同隐球菌分离株的标准化酵母悬液作为模板进行CAP59基因扩增。该方法快速、简单且成本低廉，无需PCR步骤。这对于配备了分子生物学工具的实验室进行分类学研究很重要。
Zotero 插件