Pnc1p 支持细胞内 NAD(H)水平的增加，以应对内部或外部氧化应激。
Pnc1p supports increases in cellular NAD(H) levels in response to internal or external oxidative stress.
机构信息
Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 77229-3900, USA.
出版信息
Biochemistry. 2010 Aug 3;49(30):6299-301. doi: 10.1021/bi100825z.
Abstract
Following transfer from medium with fermentable glucose to medium with nonfermentable acetate as the carbon source, cellular levels of NAD(H) were found to increase approximately 2-fold in a parental yeast strain. Similar transfer of a mutant strain subject to endogenous oxidative stress under these conditions produced more dramatic increases in cellular levels of NAD(H), and elevations above parental levels were shown to be due to the nicotinimidase Pnc1p. Similar transient increases in NAD(H) levels observed in the parental strain following addition of exogenous hydrogen peroxide were also attributable to Pnc1p.
摘要
从可发酵葡萄糖的培养基转移到不可发酵的醋酸盐作为碳源的培养基后，发现亲本酵母菌株的细胞内 NAD(H)水平增加了约 2 倍。在这些条件下，经历内源性氧化应激的突变菌株的类似转移产生了细胞内 NAD(H)水平更显著的增加，并且观察到的升高超过亲本水平是由于烟酰胺酶 Pnc1p。在外源过氧化氢添加后，亲本菌株中也观察到类似的 NAD(H)水平的短暂增加，这也归因于 Pnc1p。
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