葡萄糖恒化器，一种葡萄糖控制的连续培养系统。
Glucose-stat, a glucose-controlled continuous culture.
作者信息
Kleman G L, Chalmers J J, Luli G W, Strohl W R
机构信息
Department of Microbiology, Ohio State University, Columbus 43210.
出版信息
Appl Environ Microbiol. 1991 Apr;57(4):918-23. doi: 10.1128/aem.57.4.918-923.1991.
A predictive and feedback proportional control algorithm, developed for fed-batch fermentations and described in a companion paper (G. L. Kleman, J. J. Chalmers, G. W. Luli, and W. R. Strohl, Appl. Environ. Microbiol. 57:910-917, 1991), was used in this work to control a continuous culture on the basis of the soluble-glucose concentration (called the glucose-stat). This glucose-controlled continuous-culture system was found to reach and maintain steady state for 11 to 24 residence times when four different background glucose concentrations (0.27, 0.50, 0.7, and 1.5 g/liter) were used. The predictive-plus-feedback control system yielded very tight control of the continuous nutristat cultures; glucose concentrations were maintained at the set points with less than 0.003 standard error. Acetate production by Escherichia coli B in glucose-stats was found not to be correlated with the level of steady-state soluble-glucose concentration.
一种为分批补料发酵开发的预测与反馈比例控制算法，已在一篇配套论文（G.L. 克莱曼、J.J. 查尔默斯、G.W. 卢利和W.R. 斯特罗尔，《应用与环境微生物学》57:910 - 917, 1991）中描述，在本研究中用于基于可溶性葡萄糖浓度控制连续培养（称为葡萄糖恒化器）。当使用四种不同的背景葡萄糖浓度（0.27、0.50、0.7和1.5克/升）时，发现这个葡萄糖控制的连续培养系统在11至24个停留时间内达到并维持稳态。预测加反馈控制系统对连续营养恒化器培养实现了非常精确的控制；葡萄糖浓度维持在设定点，标准误差小于0.003。发现在葡萄糖恒化器中大肠杆菌B的乙酸盐产量与稳态可溶性葡萄糖浓度水平无关。
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