促红细胞生成素可增加自发性高血压大鼠血小板的胞浆游离钙浓度以及凝血酶诱导的胞浆游离钙变化。

Erythropoietin increases cytosolic free calcium concentration and thrombin induced changes in cytosolic free calcium in platelets from spontaneously hypertensive rats.

作者信息

Tepel M, Wischniowski H, Zidek W

机构信息

Med. Univ.-Poliklinik, University of Muenster, Germany.

出版信息

Biochem Biophys Res Commun. 1991 Jun 28;177(3):991-7. doi: 10.1016/0006-291x(91)90636-l.

DOI:10.1016/0006-291x(91)90636-l

PMID:2059226

Abstract

Using fura-2 cytosolic free calcium concentrations were measured in intact washed platelets from 9 spontaneously hypertensive rats (SHR) and from 9 age-matched normotensive Wistar-Kyoto rats (WKY). In resting platelets cytosolic free calcium concentration was significantly higher in SHR than in WKY (171.8 +/- 64.4 nM vs 93.1 +/- 59.0 nM, p less than 0.05). After preincubation with erythropoietin cytosolic free calcium concentration was significantly higher in SHR than in WKY (197.5 +/- 83.2 vs 93.0 +/- 60.1, p less than 0.01). Using platelets from SHR erythropoietin increased mean resting cytosolic free calcium concentration by 14.9% (p less than 0.05) and mean thrombin induced changes of cytosolic free calcium by 58.3% (p less than 0.01). In contrast, erythropoietin caused no significant increase in the resting calcium concentration or in thrombin induced changes of cytosolic free calcium in platelets from WKY. It is concluded that erythropoietin is involved in the pathogenesis of hypertension by elevating cytosolic free calcium concentration.

摘要

使用fura-2测量了9只自发性高血压大鼠（SHR）和9只年龄匹配的血压正常的Wistar-Kyoto大鼠（WKY）的完整洗涤血小板中的胞质游离钙浓度。在静息血小板中，SHR的胞质游离钙浓度显著高于WKY（171.8±64.4 nM对93.1±59.0 nM，p<0.05）。与促红细胞生成素预孵育后，SHR的胞质游离钙浓度显著高于WKY（197.5±83.2对93.0±60.1，p<0.01）。使用SHR的血小板，促红细胞生成素使静息胞质游离钙浓度平均增加14.9%（p<0.05），使凝血酶诱导的胞质游离钙变化平均增加58.3%（p<0.01）。相比之下，促红细胞生成素对WKY血小板的静息钙浓度或凝血酶诱导的胞质游离钙变化没有显著增加。结论是促红细胞生成素通过升高胞质游离钙浓度参与高血压的发病机制。