Marzi I, Walcher F, Menger M, Bühren V, Harbauer G, Trentz O
Chirurgische Universitätsklinik, Homburg/Saar, Federal Republic of Germany.
Transpl Int. 1991 Apr;4(1):45-50. doi: 10.1007/BF00335515.
Integrity of the hepatic microcirculation and maintenance of endothelial cell viability are critical components in preventing primary non-function after liver transplantation. Therefore, hepatic microcirculation and leucocyte-endothelial interaction were studied in rat livers stored for 1 h in Euro-Collins (EC), University of Wisconsin (UW), and histidine-tryptophan-ketoglutarate (HTK) solutions and subsequently transplanted. One hour after transplantation surgery, the livers were exposed under an intravital fluorescence microscope. After injection of the leucocyte marker acridine orange (1 mumol/kg), six pericentral fields were observed for 30 s and experiments were recorded continuously. The percentage of perfused sinusoids was reduced in the livers in the EC group (82.9%) in contrast to the UW (93.2%) and HTK groups (91.0%). Livers in the EC group showed a reduction in the diameters of pericentral sinusoids (7.3 +/- 0.2 microns; mean +/- SEM) compared with the UW group (9.5 +/- 0.2 microns; P less than 0.05) and HTK group (10.2 +/- 0.8 microns; P less than 0.05), indicating substantial cell swelling in livers stored in EC solution. Permanent adherence of leucocytes was most frequently observed in the EC group (33.5 +/- 1%), while this phenomenon was less pronounced in the UW group (14.5 +/- 1.1%; P less than 0.05) and HTK group (16.3 +/- 0.7%; P less than 0.05). Conversely, temporary adherence of leucocytes was reduced in the EC group (19.7 + 1.3%) compared with the UW group (30.5 + 2.1%) and the HTK group (34.4 + 0.8%).(ABSTRACT TRUNCATED AT 250 WORDS)
肝微循环的完整性和内皮细胞活力的维持是预防肝移植后原发性无功能的关键因素。因此，研究了在欧洲柯林斯（EC）液、威斯康星大学（UW）液和组氨酸 - 色氨酸 - 酮戊二酸（HTK）液中保存1小时后再移植的大鼠肝脏的肝微循环和白细胞与内皮细胞的相互作用。移植手术后1小时，在活体荧光显微镜下观察肝脏。注射白细胞标记物吖啶橙（1μmol/kg）后，观察六个中央周围区域30秒，并持续记录实验。与UW组（93.2%）和HTK组（91.0%）相比，EC组肝脏中灌注的肝血窦百分比降低（82.9%）。与UW组（9.5±0.2微米；P<0.05）和HTK组（10.2±0.8微米；P<0.05）相比，EC组肝脏中央周围肝血窦直径减小（7.3±0.2微米；平均值±标准误），表明储存在EC液中的肝脏存在明显的细胞肿胀。白细胞的永久性黏附在EC组中最常见（33.5±1%），而在UW组（14.5±1.1%；P<0.05）和HTK组（16.3±0.7%；P<0.05）中这种现象不太明显。相反，与UW组（30.5±2.1%）和HTK组（34.4±0.8%）相比，EC组中白细胞的暂时性黏附减少（19.7±1.3%）。（摘要截短于250字）
