Department of Microbiology, Tumor, and Cell Biology (MTC), Karolinska Institute, 171 77, Stockholm, Sweden.
Cell Mol Life Sci. 2010 Dec;67(24):4249-56. doi: 10.1007/s00018-010-0441-4. Epub 2010 Jul 1.
Epstein-Barr virus (EBV) is a human gamma herpes virus that infects B cells and induces their transformation into immortalized lymphoblasts that can grow as cell lines (LCLs) in vitro. EBNA-3 is a member of the EBNA-3-protein family that can regulate transcription of cellular and viral genes. The identification of EBNA-3 cellular partners and a study of its influence on cellular pathways are important for understanding the transforming action of the virus. In this work, we have identified the vitamin D receptor (VDR) protein as a binding partner of EBNA-3. We found that EBNA3 blocks the activation of VDR-dependent genes and protects LCLs against vitamin-D3-induced growth arrest and/or apoptosis. The presented data shed some light on the anti-apoptotic EBV program and the role of the EBNA-3-VDR interaction in the viral strategy.
EB 病毒(EBV)是一种人类γ疱疹病毒,感染 B 细胞并诱导其转化为永生的淋巴母细胞,可在体外作为细胞系(LCL)生长。EBNA-3 是 EBNA-3 蛋白家族的成员,可调节细胞和病毒基因的转录。鉴定 EBNA-3 的细胞伴侣并研究其对细胞途径的影响,对于了解病毒的转化作用很重要。在这项工作中,我们已经确定了维生素 D 受体(VDR)蛋白是 EBNA-3 的结合伴侣。我们发现 EBNA3 阻止 VDR 依赖性基因的激活,并保护 LCL 免受维生素 D3 诱导的生长停滞和/或凋亡。提出的数据阐明了 EBV 的抗凋亡程序以及 EBNA-3-VDR 相互作用在病毒策略中的作用。