Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Exp Parasitol. 2010 Dec;126(4):611-6. doi: 10.1016/j.exppara.2010.06.004. Epub 2010 Jun 8.
In this study, a cDNA encoding cyclophilin (CyP) of Gnathostoma spinigerum was cloned into a prokaryotic expression vector and expressed in Escherichia coli. The predicted molecular mass of the putative protein was 18.6kDa, and the deduced amino acid sequence had 86, 84.8, 81.3 and 77.2% identity with the CyP of Dirofilaria immitis, Brugia malayi, Onchocerca volvulus and Caenorhabditis elegans, respectively. A prediction of linear B-cell epitopes with high hydrophilicity and immunoblotting results indicated that the recombinant CyP has antigenicity to humans. The recombinant CyP protein reacted with human gnathostomiasis sera but not with other parasitosis or healthy control sera, suggesting that it might be useful for the serodiagnosis of human gnathostomiasis.
在这项研究中，旋毛虫亲环素（CyP）的 cDNA 被克隆到原核表达载体中，并在大肠杆菌中表达。推测的假定蛋白的分子量为 18.6kDa，推导的氨基酸序列与犬恶丝虫、马来丝虫、盘尾丝虫和秀丽隐杆线虫的 CyP 分别具有 86%、84.8%、81.3%和 77.2%的同一性。具有高亲水性的线性 B 细胞表位的预测和免疫印迹结果表明，重组 CyP 具有抗原性。重组 CyP 蛋白与人旋毛虫病血清反应，但与其他寄生虫病或健康对照血清不反应，提示它可能对人旋毛虫病的血清学诊断有用。
Zotero 插件