Laummaunwai Porntip, Sawanyawisuth Kittisak, Intapan Pewpan M, Chotmongkol Verajit, Wongkham Chaisiri, Maleewong Wanchai
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Parasitol Res. 2007 Aug;101(3):703-8. doi: 10.1007/s00436-007-0538-3. Epub 2007 Apr 29.
The immunoglobulin G class (total IgG) and subclass (IgG1, IgG2, IgG3, and IgG4) antibody responses to the spirurid worm Gnathostoma spinigerum were analyzed by immunoblotting technique for the antibodies' potential use in the serodiagnosis of human gnathostomiasis. Serum samples from patients with proven gnathostomiasis and from clinically suspected cases of gnathostomiasis with migratory swelling were tested. Sera from patients with other parasitic illnesses and from healthy volunteers were also analyzed. The total IgG antibody to an antigenic band of approximately 24 kDa from a somatic extract of G. spinigerum advanced third-stage larvae (GS24) gave the highest sensitivity (91.6%) and 87.8% specificity. Individual IgG subclass detection had a lower sensitivity than the detection of specific total IgG antibody, but IgG4 had a slightly higher specificity (93.9%). However, for cost effectiveness, we suggest that anti GS24 total IgG is sufficient for the routine serodiagnosis of human gnathostomiasis as well as for seroepidemiological studies in developing countries.
采用免疫印迹技术分析了针对旋尾线虫棘颚口线虫的免疫球蛋白G类（总IgG）和亚类（IgG1、IgG2、IgG3和IgG4）抗体反应，以评估这些抗体在人体颚口线虫病血清诊断中的潜在用途。对确诊为颚口线虫病的患者以及临床上疑似颚口线虫病且有游走性肿胀的病例的血清样本进行了检测。还分析了患有其他寄生虫病的患者和健康志愿者的血清。针对棘颚口线虫晚期第三期幼虫（GS24）虫体提取物中一条约24 kDa抗原条带的总IgG抗体具有最高的敏感性（91.6%）和87.8%的特异性。单个IgG亚类检测的敏感性低于特异性总IgG抗体检测，但IgG4的特异性略高（93.9%）。然而，从成本效益考虑，我们建议抗GS24总IgG足以用于人体颚口线虫病的常规血清诊断以及发展中国家的血清流行病学研究。
