Patt S, Weigel K, Zimmer C, Schreiner C
Institut für Neuropathologie, Universitätsklinikum Steglitz, Freien Universität Berlin, FRG.
Zentralbl Pathol. 1991;137(1):35-40.
The smear preparation technique is usually employed intraoperatively for stereotaxic brain tumor biopsies, whereas the frozen section technique is used in conventional operations, where larger tissue specimens can be obtained. 130 brain tumor biopsies were processed with the frozen section technique and postoperatively submitted to immunohistochemical examination. Subsequent paraffin embedding was not performed. In our study, frozen section diagnosis corresponded in 84% with the immunohistochemistry. Broggi et al. (1984) and Kleihues et al. (1984) demonstrated a similar positive correlation between intraoperative smear preparations and postoperative paraffin embedding. In our investigation, the greatest difficulties arose in cases of suspected lymphoma. Postoperatively, all lymphomas could be immunohistochemically clarified. Due to insufficient material 8% of the cases remained unclarified. Compared to smear preparation, the intraoperative frozen section technique during intervention provided a more accurate assessment of the histology. Furthermore the time needed for the procedure is not much longer than for smear preparation techniques. Reliable antibodies can be used in the remaining frozen material for further differentiation.
涂片制备技术通常在术中用于立体定向脑肿瘤活检，而冷冻切片技术则用于传统手术，在传统手术中可以获取更大的组织标本。对130例脑肿瘤活检采用冷冻切片技术处理，并在术后进行免疫组化检查。随后未进行石蜡包埋。在我们的研究中，冷冻切片诊断与免疫组化的符合率为84%。Broggi等人（1984年）和Kleihues等人（1984年）证明术中涂片制备与术后石蜡包埋之间存在类似的正相关。在我们的研究中，疑似淋巴瘤的病例出现的困难最大。术后，所有淋巴瘤都可以通过免疫组化明确诊断。由于材料不足，8%的病例仍未明确诊断。与涂片制备相比，术中冷冻切片技术在干预过程中能对组织学进行更准确的评估。此外，该操作所需时间并不比涂片制备技术长得多。可靠的抗体可用于剩余的冷冻材料进行进一步鉴别。
