Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan.
FEMS Yeast Res. 2010 Nov;10(7):948-56. doi: 10.1111/j.1567-1364.2010.00652.x. Epub 2010 Jun 28.
Five novel yeast species belonging to the Kodamaea clade are proposed in this paper based on nine strains isolated from mushrooms and soil in Taiwan between 2007 and 2008. These nine yeast strains were found to represent five novel yeast species based on their D1/D2 domain sequences of the large subunit (LSU) rRNA gene and physiological characteristics. Sequence analysis of the D1/D2 domain of the LSU rRNA gene revealed that the five species are phylogenetically related to Kodamaea and Candida sp. clustered into the Kodamaea clade in the phylogenetic tree. All the species reproduced asexually, and no ascospores could be found. Therefore, the scientific names of Candida alishanica sp. nov., Candida hsintzibuensis sp. nov., Candida kaohsiungensis sp. nov., Candida lidongshanica sp. nov., and Candida smagusa sp. nov. were proposed to accommodate these strains. Strains of the first species were discovered from soil; two strains of the second species were isolated from soil and the fruiting body of a mushroom, respectively; and strains of the latter three species were recovered from fruiting bodies of mushrooms. The type strains are listed as follows: C. alishanica GY43S11(T) (=CBS 11429(T) =BCRC 23188(T) ); C. hsintzibuensis EJ7S06(T) (=CBS 11427(T) =BCRC 23183(T) ); C. kaohsiungensis GJ10M01(T) (=CBS 11435(T) =BCRC 23197(T) ); C. lidongshanica SD5S01(T) (=CBS 11426(T) =BCRC 23191(T) ); and C. smagusa EN4M03(T) (=CBS 11430(T) =BCRC 23186(T) ).
基于 2007 年至 2008 年间在台湾从蘑菇和土壤中分离得到的 9 株菌，本研究提出了属于 Kodamaea 分支的 5 个酵母新种。这 9 株酵母菌株根据其大亚基（LSU）rRNA 基因的 D1/D2 结构域序列和生理特性，被鉴定为 5 个新的酵母种。LSU rRNA 基因 D1/D2 结构域序列分析表明，这 5 个种与 Kodamaea 和 Candida sp. 在系统发育树上聚为 Kodamaea 分支。所有种均以无性方式繁殖，未发现有性孢子。因此，将 Candida alishanica sp. nov.、Candida hsintzibuensis sp. nov.、Candida kaohsiungensis sp. nov.、Candida lidongshanica sp. nov. 和 Candida smagusa sp. nov. 等科学名称用于这些菌株。第一个种的菌株来自土壤；第二个种的两个菌株分别从土壤和蘑菇子实体中分离得到；而后三个种的菌株则从蘑菇子实体中回收得到。模式菌株如下：C. alishanica GY43S11(T) (=CBS 11429(T) =BCRC 23188(T) )；C. hsintzibuensis EJ7S06(T) (=CBS 11427(T) =BCRC 23183(T) )；C. kaohsiungensis GJ10M01(T) (=CBS 11435(T) =BCRC 23197(T) )；C. lidongshanica SD5S01(T) (=CBS 11426(T) =BCRC 23191(T) )；和 C. smagusa EN4M03(T) (=CBS 11430(T) =BCRC 23186(T) )。
