Reparto di Genomica, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Brescia 25124, Italy.
Vaccine. 2010 Jul 26;28(33):5414-20. doi: 10.1016/j.vaccine.2010.06.017. Epub 2010 Jun 18.
Myxoma virus is a poxvirus responsible for myxomatosis in European Rabbits (Oryctolagus cuniculus). The entire genome of the myxoma virus has been sequenced, allowing a systemic survey of the functions of a large number of putative pathogenic factors that this virus expresses to subvert the immune and inflammatory pathways of infected rabbit hosts. In Italy, industrial rabbits are mostly vaccinated against myxomatosis using the attenuated myxoma virus strains Borghi or SG33. We have identified genetic markers specific for Borghi or SG33 vaccine strains and established a PCR-based assay that could be used to: (a) rapidly diagnose the presence of myxoma virus in infected organs; (b) discriminate between field strain-infected and vaccinated rabbits and (c) differentiate between Borghi or SG33 vaccine strain.
黏液瘤病毒是一种痘病毒，可引起欧洲兔（Oryctolagus cuniculus）的黏液瘤病。黏液瘤病毒的整个基因组已经测序，这使得对大量假定的致病因子的功能进行系统研究成为可能，这些因子表达这些因子可颠覆受感染兔宿主的免疫和炎症途径。在意大利，工业用兔主要使用减毒的黏液瘤病毒菌株 Borghi 或 SG33 接种疫苗来预防黏液瘤病。我们已经鉴定出针对 Borghi 或 SG33 疫苗株的遗传标记，并建立了一种基于 PCR 的检测方法，可用于：（a）快速诊断感染器官中是否存在黏液瘤病毒；（b）区分田间株感染和接种疫苗的兔子；（c）区分 Borghi 或 SG33 疫苗株。
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