Molecular Virology Laboratory, First Moscow State Medical University (Sechenov University), Trubetskaya 8, 119048, Moscow, Russia.
Federal Research Center for Virology and Microbiology, Bakoulova Street, B.1, 601125, Volginskiy, Russia.
Virus Genes. 2024 Oct;60(5):528-536. doi: 10.1007/s11262-024-02090-7. Epub 2024 Jul 11.
Myxoma virus (MYXV) is a double-stranded DNA-containing virus of the family Poxviridae, genus Leporipoxvirus. MYXV is an important model virus for evolutionary and immunological research and a promising oncolytic. In this study, we sequenced and analyzed two complete genomes of MYXV virus vaccine strains B-82 and Rabbivac-B, which are widely used for vaccine production in Russia. Here, we first show that MYXV vaccine strains B-82 and Rabbivac-B share a common origin with the American recombinant MYXV MAV vaccine strain. In addition, our data suggest that the MYXV B-82 and Rabbivac-B strains contain a number of genes at the 5' and 3' ends that are identical to the virulent MYXV Lausanne strain. Several unique genetic signatures were identified in the M013L, M017L, M023, and M121R genes, helping to achieve high genetic resolution between vaccine strains. Overall, these findings highlight the evolutionary flexibility of certain genes in the MYXV genome and provide insights into the molecular epidemiology of the virus and subsequent vaccine development.
粘液瘤病毒（MYXV）是痘病毒科、兔痘病毒属的双链 DNA 病毒。MYXV 是进化和免疫学研究的重要模式病毒，也是一种很有前途的溶瘤病毒。在这项研究中，我们对两种广泛用于俄罗斯疫苗生产的 MYXV 病毒疫苗株 B-82 和 Rabbivac-B 进行了测序和分析。在这里，我们首次表明，MYXV 疫苗株 B-82 和 Rabbivac-B 与美国重组 MYXV MAV 疫苗株具有共同的起源。此外，我们的数据表明，MYXV B-82 和 Rabbivac-B 株在 5'和 3'末端包含许多与强毒 Lausanne 株相同的基因。在 M013L、M017L、M023 和 M121R 基因中发现了几个独特的遗传特征，有助于在疫苗株之间实现高遗传分辨率。总的来说，这些发现强调了 MYXV 基因组中某些基因的进化灵活性，并为病毒的分子流行病学和随后的疫苗开发提供了新的见解。
