UR 1268, Biopolymères Interactions Assemblages INRA, équipe ISD, Rue de la Géraudière, 44316 Nantes, France.
J Magn Reson. 2010 Sep;206(1):171-6. doi: 10.1016/j.jmr.2010.06.009. Epub 2010 Jun 15.
There is a strong demand in the field of solid state NMR for oriented lipid supramolecular assemblies. This is mainly devoted to biophysical structural studies or materials chemistry because the NMR signal depends on the orientation. Here we report a novel system made of a fatty acid hexagonal phase which self orient in the magnetic field. The orientation occurs within the magnetic field upon cooling the system from its isotropic phase. The cylinders of the hexagonal phase are then oriented parallel to the field. We take advantage that the hexagonal phase is a gel, i.e., the orientation is maintained fixed within the sample tube to investigate the orientational dependence of the deuterium solid state NMR signal using deuterated fatty acids and D(2)O by manually rotating the sample tube within the coil probe. As expected, the oriented signal follows the low |3cos(2)theta-1| where theta is the angle between the long cylindrical axis and the field. We expect this system to be of interest in materials chemistry and structural biology.
固态 NMR 领域对各向异性脂质超分子组装体有强烈的需求。这主要致力于生物物理结构研究或材料化学，因为 NMR 信号取决于取向。在这里，我们报告了一个由脂肪酸六方相组成的新型系统，它在磁场中自取向。在将系统从各向同性相冷却到磁场中时，会发生取向。然后，六方相的圆柱平行于磁场取向。我们利用六方相是凝胶的性质，即取向在样品管内保持固定，通过在磁线圈探头内手动旋转样品管，用氘代脂肪酸和 D2O 研究氘固态 NMR 信号的各向异性依赖性。如预期的那样，取向信号遵循低|3cos(2)theta-1|，其中 theta 是长圆柱轴与磁场之间的夹角。我们预计这个系统在材料化学和结构生物学中会很有意义。
