奶牛乳腺固有和炎性多形核白细胞的细胞死亡和 CD14 表达。

Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland.

机构信息

Department of Morphology, Physiology and Veterinary Sciences, Mendel University of Agriculture and Forestry, Zemedelska 1, Brno 613 00, Czech Republic.

出版信息

Res Vet Sci. 2011 Apr;90(2):226-34. doi: 10.1016/j.rvsc.2010.06.017.

DOI:10.1016/j.rvsc.2010.06.017

PMID:20599238

Abstract

This paper investigates the association between expression of CD14 and occurrence of apoptosis in blood, resident ((RES)PMN) and inflammatory ((INF)PMN) polymorphonuclear leukocytes (PMN) from heifer mammary glands. The fresh population of (RES)PMN contained a statistically significant higher proportion of CD14+, apoptotic and necrotic cells than did populations of (INF)PMN and blood PMN. In vitro cultivation of (RES)PMN, (INF)PMN and blood PMN led to concurrent increase of apoptotic, necrotic and CD14+ cells. A positive correlation was found between the proportions of both apoptotic and necrotic PMN and CD14+ PMN as determined by three-color flow cytometry analysis. Our study confirmed that expression of CD14 in blood PMN, (RES)PMN and (INF)PMN from heifer mammary glands was accompanied by apoptosis and necrosis.

摘要

本文研究了牛乳腺血液、驻留（RES）PMN 和炎症（INF）PMN 中性粒细胞中 CD14 表达与细胞凋亡发生之间的关系。新鲜的 RESPMN 群体中 CD14+、凋亡和坏死细胞的比例明显高于 INFPMN 和血液 PMN。体外培养 RESPMN、INFPMN 和血液 PMN 导致凋亡、坏死和 CD14+细胞同时增加。通过三色流式细胞术分析发现，PMN 凋亡和坏死的比例与 CD14+PMN 之间存在正相关。本研究证实，牛乳腺血液、RESPMN 和 INFPMN 中 CD14 的表达伴随着凋亡和坏死。