银溶胶免疫比浊法测定人 IgG。
Silver sol immunoagglutination assay for determination of human IgG.
机构信息
Institute of Biochemistry, National Academy of Sciences, PSevak str 5/1, Yerevan 0014, Republic of Armenia.
出版信息
Colloids Surf B Biointerfaces. 2010 Oct 15;80(2):180-3. doi: 10.1016/j.colsurfb.2010.06.001. Epub 2010 Jun 11.
Immunoagglutination properties of silver nanoparticles sensitized with antibodies to human IgG for determination of IgG were studied. Effects of various pH on sensitization and consequent immunoagglutination process were investigated. It was shown that the best results were obtained when sensitization and immunoagglutination were conducted at isoelectric point of IgG molecule. It was demonstrated that immunoassay with colloidal silver nanoparticles provides the detection of antigen in wide range of concentrations.
研究了用针对人 IgG 的抗体敏化的银纳米粒子的免疫凝集特性，以测定 IgG。研究了不同 pH 值对敏化和随后的免疫凝集过程的影响。结果表明，当在 IgG 分子的等电点进行敏化和免疫凝集时，可获得最佳结果。结果表明，胶体银纳米粒子免疫测定法可在宽浓度范围内检测抗原。
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