Gen-Probe Diaclone SAS, 1 Boulevard A. Fleming, 25020 Besançon, France.
J Immunol Methods. 2010 Aug 31;360(1-2):96-102. doi: 10.1016/j.jim.2010.06.014. Epub 2010 Jun 25.
The human CD4+CD25+FoxP3+ regulatory T cell population (Tregs) contains both MHC class II+ and MHC class II(-) cells. MHC class II+ Tregs belong to the integrin alpha(4)beta(1)+ subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isolation of these MHC class II expressing Tregs from large leukaphereses products using magnetic microbeads that achieves a reproducible purity of more than 90% and enables the use of this small-sized Treg population in pre-clinical application and basic research.
人类 CD4+CD25+FoxP3+调节性 T 细胞群体（Tregs）包含 MHC Ⅱ类+和 MHC Ⅱ类-细胞。MHC Ⅱ类+Tregs 属于整合素 α4β1+亚群，专门执行依赖接触的抑制活性。在这里，我们提出了一种使用磁性微珠从大型白细胞分离产品中优化分离这些 MHC Ⅱ类表达 Tregs 的方法，该方法可实现超过 90%的可重复纯度，并使这种小尺寸 Treg 群体能够在临床前应用和基础研究中使用。
