Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
FEBS Lett. 2010 Aug 4;584(15):3386-91. doi: 10.1016/j.febslet.2010.06.030. Epub 2010 Jun 26.
African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demonstrate that AHSV single-stranded RNA synthesized in vitro (core transcripts) is infectious and that there are distinct primary and secondary stages of the replication cycle. Transfection with a mixture of core transcripts from two different serotypes or a mixture of core transcripts and a T7 derived transcript resulted in the recovery of reassortant viruses. Recovery of infectious ASHV from nucleic acid will benefit investigation of the virus and the generation of attenuated vaccines.
非洲马瘟病毒（AHSV）是呼肠孤病毒科呼肠孤病毒属的一员，是一种对马业有影响的昆虫媒介病原体。对 AHSV 复制和发病机制的研究受到缺乏反向遗传学的阻碍，无法对病毒基因组进行靶向突变。我们证明了在体外合成的 AHSV 单链 RNA（核心转录本）具有感染性，并且存在明显的复制周期的主要和次要阶段。用来自两种不同血清型的核心转录本混合物或核心转录本和 T7 衍生转录本混合物进行转染，导致重组病毒的恢复。从核酸中恢复感染性 AHSV 将有利于对病毒的研究和减毒疫苗的产生。
