Migliorati G, Cardinali L, Riccardi C
Institute of Pharmacology, School of Medicine, University of Perugia, Italy.
Cell Immunol. 1991 Aug;136(1):194-207. doi: 10.1016/0008-8749(91)90394-q.
We have previously shown that interleukin-2 (IL-2) is able to induce the generation of natural killer (NK) activity in bone marrow (BM) cells from mice pretreated with 5-fluorouracil. IL-2 alone could dose-dependently induce NK activity in marrow cells and interleukin-4 (IL-4) has dual effect on the NK activity in that, depending on the concentration of IL-2, IL-4 inhibits or stimulates development of NK cells. The inhibitory effect was in part antagonized by interleukin-1 alpha. These effects were not obtained when NK-reactive spleen cells were cultured with the same concentrations of IL-2 or IL-2 plus IL-4 with or without irradiated BM cells as feeders. The effects of IL-4 were also obtained by preincubation for 6-24 hr before culturing with IL-2 alone and correlated with the expression of interleukin-2 receptor (IL-2/r), suggesting that IL-4 might play a regulatory role in the IL-2-dependent generation of NK cells in BM.
我们先前已表明，白细胞介素-2（IL-2）能够在经5-氟尿嘧啶预处理的小鼠骨髓（BM）细胞中诱导自然杀伤（NK）活性的产生。单独的IL-2可剂量依赖性地诱导骨髓细胞中的NK活性，而白细胞介素-4（IL-4）对NK活性具有双重作用，即根据IL-2的浓度，IL-4可抑制或刺激NK细胞的发育。白细胞介素-1α部分拮抗了这种抑制作用。当用相同浓度的IL-2或IL-2加IL-4培养NK反应性脾细胞，无论有无经辐照的BM细胞作为饲养细胞时，都未获得这些效果。通过在单独用IL-2培养之前预孵育6 - 24小时也可获得IL-4的效果，且这与白细胞介素-2受体（IL-2/r）的表达相关，表明IL-4可能在BM中IL-2依赖性NK细胞的产生中发挥调节作用。
