Medical University of Vienna, Department of Paediatrics and Adolescent Medicine, Vienna, Austria.
Mol Cell Endocrinol. 2010 Oct 7;327(1-2):65-71. doi: 10.1016/j.mce.2010.06.005. Epub 2010 Jun 25.
IGF-I and IGF-II are key regulators of growth and metabolism. Still, data about their expression and distribution within the growth plate in different animal models remain contradictory. Inferences drawn from rodent animal models can only be applied to human conditions to a limited extent as the rodent's growth plate never fuses. In this study, we compared the expression of IGF-I and IGF-II in native growth plates of prepubertal piglets and under different cell culture conditions. We detected IGF-I mRNA expression and abundantly expressed IGF-II within the growth plate. IGF-I expression increased during monolayer cell culture while IGF-II expression dramatically decreased. Our studies revealed that these expression patterns remained unaffected by growth hormone stimulation in vitro. The abundant expression of IGF-II in porcine growth plate tissue, both on the mRNA and on the protein level, suggests that IGF-II also has a role in growth regulation at the early postnatal stage.
IGF-I 和 IGF-II 是生长和代谢的关键调节剂。然而，关于它们在不同动物模型中的生长板内表达和分布的数据仍然存在矛盾。从啮齿动物动物模型中得出的推论只能在一定程度上应用于人类情况，因为啮齿动物的生长板永远不会融合。在这项研究中，我们比较了青春期前仔猪的天然生长板和不同细胞培养条件下 IGF-I 和 IGF-II 的表达。我们检测到生长板内 IGF-I mRNA 表达和丰富的 IGF-II 表达。IGF-I 表达在单层细胞培养期间增加，而 IGF-II 表达显著降低。我们的研究表明，这些表达模式在体外生长激素刺激下不受影响。猪生长板组织中 IGF-II 的丰富表达，无论是在 mRNA 还是蛋白水平上，都表明 IGF-II 在出生后早期的生长调节中也具有作用。
