Department of Experimental Biomedicine and Clinical Neurosciences, Human Physiology Section, University of Palermo, Corso Tukory 129, 90134 Palermo, Italy.
Neurosci Lett. 2010 Sep 6;481(2):82-7. doi: 10.1016/j.neulet.2010.06.056. Epub 2010 Jun 25.
Aim of present research was to assess, by means of descriptive and multivariate approaches, in Wistar rats tested in hole-board apparatus, whether a microstructure of hole-exploratory behavior may be identified in the behavioral response to anxiety. Two groups of 15 subjects were tested: one received 1 ml of saline IP and one was IP injected with diazepam at 0.5 mg/kg dissolved in 1 ml of saline. Following saline, six not yet reported hole-exploratory components were identified: active-dip, static-dip, rapid-dip, circular-sniff, point-sniff and central-sniff. Diazepam provoked significant changes of their per cent distribution. Results of multivariate analyses revealed a complex microstructure of hole-exploratory elements characterized by behavioral flows between sniffing and dipping patterns. Diazepam significantly modified several patternings, mainly those involving point-sniff and static-dip. Hence, the hypothesis of an important role of such behavioral elements and of their patternings, in anxiety-related hole-exploratory behavior, can be proposed. It is suggested that modifications of point-sniff and static-dip patternings could evidence even subtle changes of animal anxiety condition.
本研究旨在通过描述性和多变量方法评估，在洞板仪器中测试的 Wistar 大鼠，是否可以确定行为反应焦虑中的孔探索行为的微观结构。两组 15 只动物接受了测试：一组腹腔注射 1 毫升生理盐水，另一组腹腔注射 0.5 毫克/公斤的安定，溶解在 1 毫升生理盐水中。在生理盐水后，确定了六个尚未报告的孔探索成分：主动浸、静态浸、快速浸、圆形嗅探、点状嗅探和中央嗅探。安定引起了它们的百分比分布的显著变化。多变量分析的结果显示了孔探索元素的复杂微观结构，其特征是嗅探和浸探模式之间的行为流动。安定显著改变了几种模式，主要是那些涉及点状嗅探和静态浸。因此，可以提出这样的假设，即这些行为元素及其模式在与焦虑相关的孔探索行为中起着重要作用。建议改变点状嗅探和静态浸的模式可以证明动物焦虑状况的甚至细微变化。
